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Methods in Molecular Biology

Recombinant Gene Expression

Reviews and Protocols

Editors: Balbas, Paulina, Lorence, Argelia (Eds.)

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eBook $149.00
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  • ISBN 978-1-59259-774-1
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Hardcover $199.00
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  • ISBN 978-1-58829-262-9
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Softcover $199.00
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  • ISBN 978-1-61737-460-9
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

In this updated and expanded second edition of an established classic, the editors have added critical reviews to a fresh collection of cutting-edge protocols for gene expression in bacteria, fungi, plants, plant cells, animals, and animal cells. The review articles survey new directions in recombinant gene expression research, technique, and application, and point the way to using recombinant gene expression for metabolic engineering and the production of nonprotein molecules. The readily reproducible protocols offer new host-vector systems, such as Antarctic bacteria and moderately halophytic bacteria, vectors for chromosomal editing and metabolic engineering, and information to aid in problem solving, strategy planning, and the promotion of protein folding. There are also novel heterologous gene expression schemes for cell-free systems, plant chloroplasts, microplates, hairy roots, and animal milk, and new developments in consolidated heterologous systems. The protocols follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
State-of-the-art and forward looking, Recombinant Gene Expression: Reviews and Protocols, Second Edition offers investigators seeking an overview of this critically important field not only the understanding, but also the tools needed to begin producing nonprotein products and altering the central metabolic pathways of cells to enhance heterologous gene expression.

Reviews

From reviews of the first edition...
"...particularly useful for teaching molecular biology laboratory courses."
-Quarterly Review of Biology

"...represents good value and will be a useful addition to the laboratory."
-Microbiology Today

Table of contents (33 chapters)

  • Host Cell Compatibility in Protein Expression

    Greene, James J.

    Pages 3-14

  • Production of Recombinant Proteins

    Palomares, Laura A. (et al.)

    Pages 15-51

  • Folding-Promoting Agents in Recombinant Protein Production

    Fahnert, Beatrix

    Pages 53-74

  • Back to Basics

    Balbás, Paulina (et al.)

    Pages 77-90

  • α-Complementation-Enabled T7 Expression Vectors and Their Use for the Expression of Recombinant Polypeptides for Protein Transduction Experiments

    Alexeyev, Mikhail F. (et al.)

    Pages 91-100

Buy this book

eBook $149.00
price for USA in USD (gross)
  • ISBN 978-1-59259-774-1
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $199.00
price for USA in USD
  • ISBN 978-1-58829-262-9
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $199.00
price for USA in USD
  • ISBN 978-1-61737-460-9
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Recombinant Gene Expression
Book Subtitle
Reviews and Protocols
Editors
  • Paulina Balbas
  • Argelia Lorence
Series Title
Methods in Molecular Biology
Series Volume
267
Copyright
2004
Publisher
Humana Press
Copyright Holder
Humana Press
eBook ISBN
978-1-59259-774-1
DOI
10.1385/1592597742
Hardcover ISBN
978-1-58829-262-9
Softcover ISBN
978-1-61737-460-9
Series ISSN
1064-3745
Edition Number
2
Number of Pages
XVI, 508
Topics