Overview
- Editors:
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Paulina Balbás
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Centro de Investigación en Biotecnología, UAEM, Cuernavaca, México
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Argelia Lorence
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Virginia Polytechnic Institute and State University, Blacksburg
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Table of contents (33 protocols)
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Fungi
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- Kevin J. Verstrepen, Johan M. Thevelein
Pages 259-265
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- Caterina Holz, Christine Lang
Pages 267-276
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- Mewes Böttner, Christine Lang
Pages 277-286
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- Björn Johansson, Bärbel Hahn-Hägerdal
Pages 287-296
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- Vianey Olmedo-Monfil, Carlos Cortés-Penagos, Alfredo Herrera-Estrella
Pages 297-313
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- Sergio Casas-Flores, Teresa Rosales-Saavedra, Alfredo Herrera-Estrella
Pages 315-325
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Plants and Plant Cells
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Front Matter
Pages 327-327
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- Argelia Lorence, Robert Verpoorte
Pages 329-350
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- Fabricio Medina-Bolívar, Carole Cramer
Pages 351-363
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- Shashi Kumar, Henry Daniell
Pages 365-383
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- Barbara Leyman, Nelson Avonce, Matthew Ramon, Patrick Van Dijck, Johan M. Thevelein, Gabriel Iturriaga
Pages 385-396
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- Gerardo Acosta-García, Daphné Autran, Jean-Philippe Vielle-Calzada
Pages 397-414
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Animals and Animal Cells
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Front Matter
Pages 415-415
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- Héctor Rincón-Arano, Félix Recillas-Targa
Pages 435-450
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- Wilfried Weber, Martin Fussenegger
Pages 451-466
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- Dagmar Wirth, Hansjörg Hauser
Pages 467-476
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- Jean-Louis Goergen, Lucía Monaco
Pages 477-483
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Back Matter
Pages 495-506
About this book
Since newly created beings are often perceived as either wholly good or bad, the genetic alteration of living cells impacts directly on a symbolic meaning deeply imbedded in every culture. During the earlier years of gene expression research, te- nological applications were confined mainly to academic and industrial laboratories, and were perceived as highly beneficial since molecules that were previously unable to be separated or synthesized became accessible as therapeutic agents. Such were the success stories of hormones, antibodies, and vaccines produced in the bacterium Escherichia coli. Originally this bacterium gained fame among humans for being an unwanted host in the intestine, or worse yet, for being occasionally dangerous and pathogenic. H- ever, it was easily identified in contaminated waters during the 19th century, thus becoming a clear indicator of water pollution by human feces. Tamed, cultivated, and easily maintained in laboratories, its fast growth rate and metabolic capacity to adjust to changing environments fascinated the minds of scientists who studied and modeled such complex phenomena as growth, evolution, genetic exchange, infection, survival, adaptation, and further on—gene expression. Although at the lower end of the complexity scale, this microbe became a very successful model system and a key player in the fantastic revolution kindled by the birth of recombinant DNA technology.
Reviews
From reviews of the first edition...
"...particularly useful for teaching molecular biology laboratory courses."
-Quarterly Review of Biology
"...represents good value and will be a useful addition to the laboratory."
-Microbiology Today
Editors and Affiliations
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Centro de Investigación en Biotecnología, UAEM, Cuernavaca, México
Paulina Balbás
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Virginia Polytechnic Institute and State University, Blacksburg
Argelia Lorence