Methods in Molecular Biology

Mass Spectrometry-Based Lipidomics

Methods and Protocols

Editors: Hsu, Fong-Fu (Ed.)

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts
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  • ISBN 978-1-0716-1410-5
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About this book

This detailed volume covers conventional MS-based “shotgun lipidomics” by which samples are introduced by infusion or loop injection, as well as LC-MS-based lipidomics, which are becoming increasingly important due to the ever-increasing demand for a complete and precise lipid analysis of the complex and diversified lipids in nature. The volume features protocols applying chemical reactions, the on-line photochemical reactions combined with various MS methods for comprehensive characterization of various lipid classes, and quantification of specific and rare lipids. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 
Authoritative and practical, Mass Spectrometry-Based Lipidomics: Methods and Protocols serves as an invaluable guide for biochemists and mass spectroscopists who are interested in lipid studies.

Table of contents (20 chapters)

Table of contents (20 chapters)
  • Mass Spectrometry-Based Lipidomics: An Overview

    Pages 1-10

    Hsu, Fong-Fu

  • High Confidence Shotgun Lipidomics Using Structurally Selective Ion Mobility-Mass Spectrometry

    Pages 11-37

    Rose, Bailey S. (et al.)

  • Global Lipidomics Profiling by a High Resolution LC-MS Platform

    Pages 39-51

    Züllig, Thomas (et al.)

  • Comprehensive Structural Characterization of Lipids by Coupling Paternò–Büchi Reaction and Tandem Mass Spectrometry

    Pages 53-60

    Hu, Qingyuan (et al.)

  • Chemical Derivatization-Aided High Resolution Mass Spectrometry for Shotgun Lipidome Analysis

    Pages 61-75

    Hofferek, Vinzenz (et al.)

Buy this book

eBook $169.00
price for USA in USD
  • ISBN 978-1-0716-1410-5
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $219.99
price for USA in USD
  • ISBN 978-1-0716-1409-9
  • with online files
  • Free shipping for individuals worldwide
  • Institutional customers should get in touch with their account manager
  • Covid-19 shipping restrictions
  • Usually ready to be dispatched within 3 to 5 business days, if in stock
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Bibliographic Information

Bibliographic Information
Book Title
Mass Spectrometry-Based Lipidomics
Book Subtitle
Methods and Protocols
Editors
  • Fong-Fu Hsu
Series Title
Methods in Molecular Biology
Series Volume
2306
Copyright
2021
Publisher
Springer US
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-0716-1410-5
DOI
10.1007/978-1-0716-1410-5
Hardcover ISBN
978-1-0716-1409-9
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 314
Number of Illustrations
29 b/w illustrations, 62 illustrations in colour
Topics