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Mass Spectrometry-Based Lipidomics

Methods and Protocols

  • Book
  • © 2021

Overview

Editors:
  1. Fong-Fu Hsu

    1. Mass Spectrometry Resource Division of Endocrinology Metabolism, and Lipid Research Department of Medicine, Washington University School of Medicine, St. Louis, USA

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2306)

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Table of contents (20 protocols)

  1. Matrix-Assisted Laser Desorption/Ionization-Mass Spectrometry Imaging of Lipids in the Ischemic Rat Brain Section: A Practical Approach

    • Hay-Yan J. Wang
    Pages 299-311

  2. Back Matter

    Pages 313-314
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Keywords

About this book

This detailed volume covers conventional MS-based “shotgun lipidomics” by which samples are introduced by infusion or loop injection, as well as LC-MS-based lipidomics, which are becoming increasingly important due to the ever-increasing demand for a complete and precise lipid analysis of the complex and diversified lipids in nature. The volume features protocols applying chemical reactions, the on-line photochemical reactions combined with various MS methods for comprehensive characterization of various lipid classes, and quantification of specific and rare lipids. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Mass Spectrometry-Based Lipidomics: Methods and Protocols serves asan invaluable guide for biochemists and mass spectroscopists who are interested in lipid studies.

Editors and Affiliations

  • Mass Spectrometry Resource Division of Endocrinology Metabolism, and Lipid Research Department of Medicine, Washington University School of Medicine, St. Louis, USA

    Fong-Fu Hsu

Bibliographic Information

  • Book Title: Mass Spectrometry-Based Lipidomics

  • Book Subtitle: Methods and Protocols

  • Editors: Fong-Fu Hsu

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-1410-5

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2021

  • Hardcover ISBN: 978-1-0716-1409-9Published: 06 May 2021

  • Softcover ISBN: 978-1-0716-1412-9Published: 07 May 2022

  • eBook ISBN: 978-1-0716-1410-5Published: 05 May 2021

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 314

  • Number of Illustrations: 29 b/w illustrations, 62 illustrations in colour

  • Topics: Lipidology, Mass Spectrometry

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