Methods in Molecular Biology

Neurotrophic Factors

Methods and Protocols

Editors: Skaper, Stephen D. (Ed.)

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  • Includes cutting-edge methods and protocols for the study of neurotrophic factors
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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  • ISBN 978-1-4939-7571-6
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Hardcover 176,79 €
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Softcover 176,79 €
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  • ISBN 978-1-4939-8524-1
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About this book

This volume collects protocols and procedures utilizing cellular, tissue, and whole animal models that can be applied to the investigation of neurotrophic factors and other agents impacting on these systems. It begins with chapters on the culture of neurons and glia from the central and peripheral nervous systems, neuron-glia and glia-glia co-culture models, oligodendrocytes, and cell-based assays for the evaluation of cell vitality. This revised second edition expands to cover methodology encompassing site-specific direct labeling of neurotrophins and their receptors, angiogenesis assays, stem cells, pancreatic beta-cells, axonal transport, synapse biology, dendritic spine analysis, and brain endothelial cells. Subsequent chapters are dedicated to in vivo lesion models of relevance to nervous system pathology, which can be applied to the investigation of neurotrophic factors and peptides, as well as protocols describing nanofiber- and nanoparticle-based methods for brain delivery of neurotrophic agents. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 
Authoritative and practical, Neurotrophic Factors: Methods and Protocols, Second Edition is a valuable resource for a wide audience of readers as they explore nervous system function and pathology.

Table of contents (36 chapters)

Table of contents (36 chapters)
  • Neurotrophic Factors: An Overview

    Skaper, Stephen D.

    Pages 1-17

  • Ocular Nerve Growth Factor (NGF) and NGF Eye Drop Application as Paradigms to Investigate NGF Neuroprotective and Reparative Actions

    Tirassa, Paola (et al.)

    Pages 19-38

  • Culture of Rodent Cortical, Hippocampal, and Striatal Neurons

    Facci, Laura (et al.)

    Pages 39-47

  • Culture of Neonatal Rodent Microglia, Astrocytes, and Oligodendrocytes from the Cortex, Spinal Cord, and Cerebellum

    Skaper, Stephen D. (et al.)

    Pages 49-61

  • Central Nervous System Neuron-Glia co-Culture Models and Application to Neuroprotective Agents

    Skaper, Stephen D. (et al.)

    Pages 63-80

Buy this book

eBook 142,79 €
price for Spain (gross)
  • ISBN 978-1-4939-7571-6
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover 176,79 €
price for Spain (gross)
  • ISBN 978-1-4939-7570-9
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
  • The final prices may differ from the prices shown due to specifics of VAT rules
Softcover 176,79 €
price for Spain (gross)
  • ISBN 978-1-4939-8524-1
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
  • The final prices may differ from the prices shown due to specifics of VAT rules
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Bibliographic Information

Bibliographic Information
Book Title
Neurotrophic Factors
Book Subtitle
Methods and Protocols
Editors
  • Stephen D. Skaper
Series Title
Methods in Molecular Biology
Series Volume
1727
Copyright
2018
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-4939-7571-6
DOI
10.1007/978-1-4939-7571-6
Hardcover ISBN
978-1-4939-7570-9
Softcover ISBN
978-1-4939-8524-1
Series ISSN
1064-3745
Edition Number
2
Number of Pages
XV, 458
Number of Illustrations
51 b/w illustrations, 70 illustrations in colour
Topics