Overview
- Editors:
-
-
Stephen D. Skaper
-
Department of Pharmaceutical and Pharmacological Sciences, University of Padova, Padova, Italy
- Includes cutting-edge methods and protocols for the study of neurotrophic factors
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Access this book
Other ways to access
Table of contents (36 protocols)
-
- Myrian Velasco, Carlos Larqué, Carlos Manlio DÃaz-GarcÃa, Carmen Sanchez-Soto, Marcia Hiriart
Pages 261-273
-
- Micaela Pannella, Luciana Giardino, Laura Calzà , Mercedes Fernández
Pages 275-294
-
- Francesco Gobbo, Fulvio Bonsignore, Rosy Amodeo, Antonino Cattaneo, Laura Marchetti
Pages 295-314
-
- Hector Rosas-Hernandez, Elvis Cuevas, Susan M. Lantz, Merle G. Paule, Syed F. Ali
Pages 315-331
-
- Maria Rosanna Bronzuoli, Roberta Facchinetti, Caterina Scuderi
Pages 333-341
-
- Roberta Facchinetti, Maria Rosanna Bronzuoli, Caterina Scuderi
Pages 343-352
-
- Gabriella Contarini, Pietro Giusti, Stephen D. Skaper
Pages 353-360
-
- Vincenzo Sorrenti, Pietro Giusti, Morena Zusso
Pages 361-372
-
- Serena Boccella, Francesca Guida, Enza Palazzo, Ida Marabese, Vito de Novellis, Sabatino Maione et al.
Pages 373-378
-
- Irene Paterniti, Emanuela Esposito, Salvatore Cuzzocrea
Pages 379-384
-
- Michela Campolo, Emanuela Esposito, Salvatore Cuzzocrea
Pages 385-391
-
- Rosalia Crupi, Rosanna Di Paola, Emanuela Esposito, Salvatore Cuzzocrea
Pages 393-401
-
- Jun-Kyo Francis Suh, Sujin Hyung
Pages 403-411
-
-
- Rosa Espinosa-Luna, Alma R. Barajas-Espinosa, Fernando Ochoa-Cortez, Carlos Barajas-López
Pages 423-432
-
- Asya Ozkizilcik, Roger Williams, Z. Ryan Tian, Dafin F. Muresanu, Aruna Sharma, Hari S. Sharma
Pages 433-442
-
- Muge Yemisci, Secil Caban, Eduardo Fernandez-Megia, Yilmaz Capan, Patrick Couvreur, Turgay Dalkara
Pages 443-454
-
Back Matter
Pages 455-458
About this book
This volume collects protocols and procedures utilizing cellular, tissue, and whole animal models that can be applied to the investigation of neurotrophic factors and other agents impacting on these systems. It begins with chapters on the culture of neurons and glia from the central and peripheral nervous systems, neuron-glia and glia-glia co-culture models, oligodendrocytes, and cell-based assays for the evaluation of cell vitality. This revised second edition expands to cover methodology encompassing site-specific direct labeling of neurotrophins and their receptors, angiogenesis assays, stem cells, pancreatic beta-cells, axonal transport, synapse biology, dendritic spine analysis, and brain endothelial cells. Subsequent chapters are dedicated to in vivo lesion models of relevance to nervous system pathology, which can be applied to the investigation of neurotrophic factors and peptides, as well as protocols describing nanofiber- and nanoparticle-based methods for brain delivery of neurotrophic agents. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Â
Authoritative and practical, Neurotrophic Factors: Methods and Protocols, Second Edition is a valuable resource for a wide audience of readers as they explore nervous system function and pathology.
Editors and Affiliations
-
Department of Pharmaceutical and Pharmacological Sciences, University of Padova, Padova, Italy
Stephen D. Skaper