Overview
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 213)
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About this book
Recent developments in high-resolution separation techniques based on capillary-scale chromatography and electrophoresis have transformed the analysis of free and conjugated mono- and oligosaccharides. In Capillary Electrophoresis of Carbohydrates, hands-on experts describe cutting-edge techniques in capillary electrophoresis (CE) for the analysis of complex carbohydrates. Written in step-by-step detail to ensure successful experimental results, these readily reproducible protocols provide methods for sample preparation and analysis of mono- and oligosaccharides, glycoproteins, and glycoconjugates. Different CE separation formats, including capillary zone electrophoresis (CZE), capillary isoelectric focusing (CIEF), capillary isotachophoresis (CITP), capillary gel electrophoresis (CGE), and micellar electrokinetic chromatography (MEKC), are presented for different types of applications, such as the analysis of glycoforms, the separation of enantiomers, and the resolution of oligosaccharides. The separation of native derivatized carbohydrates is presented using UV, fluorescence, and mass spectrometric detection. Two other applications demonstrate the use of CE as an assay method for monitoring glycosyltransferase activity and for determining the association constant of carbohydrate-protein interaction. A useful appendix describes the structures of the most commonly encountered carbohydrate residues and oligosaccharides from mammalian and bacterial origins. Each protocol contains detailed information on reagents, apparatus, and valuable comments and notes on methods.
Timely and highly practical, Capillary Electrophoresis of Carbohydrates provides both novice and experienced CE analysts with powerful tools for the successful separation of complex carbohydrates by capillary electrophoresis.
Timely and highly practical, Capillary Electrophoresis of Carbohydrates provides both novice and experienced CE analysts with powerful tools for the successful separation of complex carbohydrates by capillary electrophoresis.
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Keywords
Table of contents (16 protocols)
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Front Matter
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Introduction
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Front Matter
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Analysis of Mono- and Oligosaccharides
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Front Matter
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Analysis of Glycoconjugates and the Component Monosaccharides
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Front Matter
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Specific Applications
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Front Matter
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Reviews
"...the book will be very valuable for researchers carrying out carbohydrate research, or planning to do so, and contemplating the best analytical approaches." -Pharmaceutical Research
"...this book provides a great deal of useful practical detail for CE analysis of carbohydrates for the experienced as well as the newcomer to the field." -Biomedical Chromatography
"...the book is a nice compilation of protocols for the analysis of carbohydrates by CE...it does contain all the essential procedures and methods needed to solve many problems." - Chromatographia
"...this book comprehensively summarizes knowledge on carbohydrate analysis by means of capillary electrophoresis." - Biochemistry
"...this book provides a great deal of useful practical detail for CE analysis of carbohydrates for the experienced as well as the newcomer to the field." -Biomedical Chromatography
"...the book is a nice compilation of protocols for the analysis of carbohydrates by CE...it does contain all the essential procedures and methods needed to solve many problems." - Chromatographia
"...this book comprehensively summarizes knowledge on carbohydrate analysis by means of capillary electrophoresis." - Biochemistry
Editors and Affiliations
Bibliographic Information
Book Title: Capillary Electrophoresis of Carbohydrates
Editors: Pierre Thibault, Susumu Honda
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1385/1592592945
Publisher: Humana Totowa, NJ
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eBook Packages: Springer Book Archive
Copyright Information: Humana Press 2003
Hardcover ISBN: 978-0-89603-826-4Published: 14 January 2003
Softcover ISBN: 978-1-61737-206-3Published: 10 November 2010
eBook ISBN: 978-1-59259-294-4Published: 03 February 2008
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XIV, 318