Overview
- Editors:
-
-
Donna H. Wang
-
Department of Medicine, Michigan State University, East Lansing
Access this book
Other ways to access
About this book
A qualitative leap in the understanding of cardiovascular and n- ral regulation by the renin–angiotensin system, and of the role of this s- tem in tissue damage, has occurred as a result of the many recent advances in molecular genetic techniques. The cloning of the genes for the components of the renin–angiotensin system, the design of specific angiotensin receptor ligands, and the use of embryonic gene targeting te- niques for the creation of mutant strains have established that the renin– angiotensin system is important in blood pressure regulation, ion and fluid homeostasis, and tissue growth and remodeling Further investigation of the mechanisms by which this system p- ticipates in cardiovascular regulation may shed some light on the pat- genesis of several cardiovascular diseases, e. g. , hypertension, congestive heart failure, and chronic renal failure. Despite the promise of this system as a target for therapeutic interventions for these diseases, there are great challenges in the integration of the attempts to close the gap between the traditional literature of medicine and the explosion of information from the new technologies. This book’s title, Angiotensin Protocols, reflects the authors’ strong efforts to translate expert knowledge into easy-to-follow practice. The book opens with introductory chapters, and each specialty section provides detailed methods covering a wide variety of techniques, ranging from genetic manipulation of targeted genes to functional studies of the renin– angiotensin system.
Table of contents (34 protocols)
-
-
Introduction to The Renin-Angiotensin System
-
-
-
- Cheryl L. Laffer, Fernando Elijovich
Pages 23-37
-
Transgenic and Gene Targeting Methods
-
-
- Junji Takaya, Taiji Matsusaka, Iekuni Ichikawa
Pages 41-52
-
- Curt D. Sigmund, David E. Stec
Pages 53-65
-
- Michael J. Katovich, Hong-Wei Wang, Craig H. Gelband, Mohan K. Raizada
Pages 67-81
-
- Dagmara Mohuczy, M. Ian Phillips
Pages 83-104
-
Analysis of Transcriptional and Translational Mechanisms
-
Front Matter
Pages 105-105
-
- Christopher T. Sherman, Allan R. Brasier
Pages 107-125
-
- Allan R. Brasier, Christopher T. Sherman, Mohammad Jamaluddin
Pages 127-150
-
- Zheng Wu, Kamakshi Krishnamurthi, Koby Mok, Kathryn Sandberg
Pages 151-170
-
- Hong Ji, Kamakshi Krishnamurthi, Zheng Wu, Kathryn Sandberg
Pages 171-192
-
Assays For Determination of Mrna Levels
-
Front Matter
Pages 193-193
-
- Wei Wu, Aqing Yao, Donna H. Wang
Pages 195-204
-
- Patricia E. Gallagher, Debra I. Diz
Pages 205-213
-
- Scott C. Supowit, Donald J. DiPette
Pages 215-222
-
- Susan Riggs Runge, Zhaoyong Hu, Marschall S. Runge
Pages 223-233
-
Assays For Determination of Protein Contents and Enzyme Activity
-
Front Matter
Pages 235-235
Reviews
"This is an advanced treatise on the renin-angiotensin system (RAS) and its regulation of the cardiovascular, renal, and neural relationships in health and disease. The introduction is ample to move the biological scientist reader up to adequacy to comprehend a complex patient example. ...The purpose shows a goal along with suitable objectives to move traditional medicine into utilization of the amazing explosion of modern biological techniques in RAS applications. It does so with a compilation of experts that foster a clearer picture of the modem RAS field. These are indeed worthy objectives, that surpass what individual papers and review articles can accomplish. Indeed it is needed and it serves as a tremendous thinking stimulator....The introduction by John E. Hall sets the tone and makes this book happen. The genetic manipulation of the RAS by Sigmund and Stec keeps the happening alive. the quantification of angiotensin II receptors by Wu, Yao and Wang is an outstanding peace of work. The cyclic AMP and GMP by Ji was helpful as was the NO from assay work of Thorup and Moore. The closing article on cerebral circulation by Lombard carries the exciting nature of the book to the interested student."-Doody's Health Sciences Book Review Journal
"This book with great attention to the recent developments in molecular genetic, biochemical and fysiological techniques can be recommended to all researchers involved in the fundemental research of the RAS at the cellular level." - Acta Cardiologica
Editors and Affiliations
-
Department of Medicine, Michigan State University, East Lansing
Donna H. Wang