Overview
- Editors:
-
-
Ian R. Phillips
-
School of Biological Sciences, Queen Mary University of London, London, UK
-
Elizabeth A. Shephard
-
Department of Biochemistry and Molecular Biology, University College London, London, UK
Access this book
Other ways to access
Table of contents (37 protocols)
-
- Catherine K. Yeung, Allan E. Rettie
Pages 157-162
-
- Catherine K. Yeung, Allan E. Rettie
Pages 163-172
-
-
- Sarah C. Sim, Magnus Ingelman-Sundberg
Pages 183-191
-
- Ann K. Daly, Barry P. King, Julian B. S. Leathart
Pages 193-207
-
- Tamara Vanhaecke, Vera Rogiers
Pages 209-227
-
- Peggy Papeleu, Tamara Vanhaecke, Tom Henkens, Greetje Elaut, Mathieu Vinken, Sarah Snykers et al.
Pages 229-237
-
- Tom Henkens, Tamara Vanhaecke, Peggy Papeleu, Greetje Elaut, Mathieu Vinken, Sarah Snykers et al.
Pages 239-246
-
- Mathieu Vinken, Greetje Elaut, Tom Henkens, Peggy Papeleu, Sarah Snykers, Tamara Vanhaecke et al.
Pages 247-254
-
- Greetje Elaut, Peggy Papeleu, Mathieu Vinken, Tom Henkens, Sarah Snykers, Tamara Vanhaecke et al.
Pages 255-263
-
- Sarah Snykers, Tamara Vanhaecke, Vera Rogiers
Pages 265-272
-
- Mina Edwards, Siew Cheng Wong, Roongsiri Chotpadiwetkul, Despina Smirlis, Ian R. Phillips, Elizabeth A. Shephard
Pages 273-282
-
- Lydiane Pichard, Edith Raulet, Gérard Fabre, Jean Bernard Ferrini, Jean-Claude Ourlin, Patrick Maurel
Pages 283-293
-
- Azara Janmohamed, Ian R. Phillips, Elizabeth A. Shephard
Pages 295-305
-
- Diana Hernandez, Pritpal Chandan, Azara Janmohamed, Ian R. Phillips, Elizabeth A. Shephard
Pages 307-319
-
- Diana Hernandez, Ian R. Phillips, Elizabeth A. Shephard
Pages 321-327
-
- Diana Hernandez, Anna N. Melidoni, Ian R. Phillips, Elizabeth A. Shephard
Pages 329-341
-
-
Back Matter
Pages 355-363
About this book
For this second edition of their much praised Cytochrome P450, the editors have collected accounts of the essential core techniques that use the latest methodologies for the investigation of P450s. Highlights include protocols for spectral analysis and purification of P450s, enzymatic assays of P450s and flavin-containing monooxygenases (FMOs), expression of P450s and FMOs in heterologous systems, and the production and use of antipeptide antibodies. Additional chapters contain readily reproducible techniques for the transfection of hepatocytes for gene regulation studies, P450 reporter gene assays, in situ hybridization, and analysis of genetic polymorphisms. Although the emphasis is on P450s of mammalian origin, many of the readily reproducible methods described are suitable for P450s from any source.
Reviews
From Reviews of the First Edition...
"...a remarkable book, highly useful for all our scientific readers..." -Cellular and Molecular Biology
"...will be welcomed by laboratory scientists of various profiles who need authoritative protocols for their studies." -Modern Pathology
From the reviews of the second edition:
"In this second edition, editors Phillips and Shephard have updated the text to reflect current advances and to include chapters on some more contemporary procedures … . I’m sure that the wealth of background information in the book would be valuable. Overall … this is a useful reference book for experienced researchers and for those new to this discipline. Recommended." (David Wilkinson, British Toxicology Society Newsletter, Winter, 2006)
Editors and Affiliations
-
School of Biological Sciences, Queen Mary University of London, London, UK
Ian R. Phillips
-
Department of Biochemistry and Molecular Biology, University College London, London, UK
Elizabeth A. Shephard