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Protein'Ligand Interactions

Methods and Applications

  • Book
  • © 2005

Overview

Editors:
  1. G. Ulrich Nienhaus

    1. Department of Biophysics, University of Ulm, Ulm, Germany

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Part of the book series: Methods in Molecular Biology (MIMB, volume 305)

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About this book

A readily reproducible collection of established and emerging techniques for studying the interaction between proteins and ligands, including biochemical/bulk techniques, structure analysis, spectroscopy, single-molecule studies, and theoretical/computational tools. Among the highlights are surface plasmon resonance (SPR) and reflectometric biosensor approaches, high-throughput screening with confocal optics microscopy, single molecule fluorescence and fluorescence correlation spectroscopy (FCS), atomic force microscopy (AFM), crystallography of reaction intermediates, and time-resolved x-ray crystallography. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

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Table of contents (24 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Isothermal Titration Calorimetry

    • Edwin A. Lewis, Kenneth P. Murphy
    Pages 1-15

  3. Direct Optical Detection of Protein-Ligand Interactions

    • Frank Gesellchen, Bastian Zimmermann, Friedrich W. Herberg
    Pages 17-45

  4. Label-Free Detection of Protein-Ligand Interactions by the Quartz Crystal Microbalance

    • Andreas Janshoff, Claudia Steinem
    Pages 47-63

  5. Measurement of Solvent Accessibility at Protein-Protein Interfaces

    • Jeffrey G. Mandell, Abel Baerga-Ortiz, Arnold M. Falick, Elizabeth A. Komives
    Pages 65-79

  6. Hydrophobic Interaction Chromatography

    • Herbert P. Jennissen
    Pages 81-99

  7. Sedimentation Velocity Method in the Analytical Ultracentrifuge for the Study of Protein-Protein Interactions

    • Claus Urbanke, Gregor Witte, Ute Curth
    Pages 101-113

  8. Protein-Ligand Interaction Probed by Time-Resolved Crystallography

    • Marius Schmidt, Hyotcherl Ihee, Reinhard Pahl, Vukica Šrajer
    Pages 115-154

  9. X-Ray Crystallography of Protein-Ligand Interactions

    • Ilme Schlichting
    Pages 155-165

  10. Combined Use of XAFS and Crystallography for Studying Protein-Ligand Interactions in Metalloproteins

    • Richard W. Strange, S. Samar Hasnain
    Pages 167-196

  11. NMR Studies of Protein-Ligand Interactions

    • Till Maurer
    Pages 197-213

  12. Probing Heme Protein-Ligand Interactions by UV/Visible Absorption Spectroscopy

    • Karin Nienhaus, G. Ulrich Nienhaus
    Pages 215-241

  13. Ultrafast Time-Resolved IR Studies of Protein-Ligand Interactions

    • Manho Lim, Philip A. Anfinrud
    Pages 243-259

  14. Monitoring Protein-Ligand Interactions by Time-Resolved FTIR Difference Spectroscopy

    • Carsten Kötting, Klaus Gerwert
    Pages 261-285

  15. Proteins in Motion

    • Uri Samuni, Joel M. Friedman
    Pages 287-300

  16. Fluorescence Polarization/Anisotropy Approaches to Study Protein-Ligand Interactions

    • David M. Jameson, Gabor Mocz
    Pages 301-322

  17. Ligand Binding With Stopped-Flow Rapid Mixing

    • Mark S. Hargrove
    Pages 323-341

  18. Circular Dichroism Spectroscopy for the Study of Protein-Ligand Interactions

    • Alison Rodger, Rachel Marrington, David Roper, Stuart Windsor
    Pages 343-363

  19. High-Throughput Screening of Interactions Between G ProteinCoupled Receptors and Ligands Using Confocal Optics Microscopy

    • Lenka Zemanová, Andreas Schenk, Martin J. Valler, G. Ulrich Nienhaus, Ralf Heilker
    Pages 365-383

  20. Single-Molecule Study of Protein-Protein and Protein-DNA Interaction Dynamics

    • H. Peter Lu
    Pages 385-413
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Editors and Affiliations

  • Department of Biophysics, University of Ulm, Ulm, Germany

    G. Ulrich Nienhaus

Bibliographic Information

  • Book Title: Protein'Ligand Interactions

  • Book Subtitle: Methods and Applications

  • Editors: G. Ulrich Nienhaus

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/1592599125

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2005

  • Hardcover ISBN: 978-1-58829-372-5Published: 21 March 2005

  • Softcover ISBN: 978-1-61737-525-5Published: 19 November 2010

  • eBook ISBN: 978-1-59259-912-7Published: 02 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 568

  • Topics: Biochemistry, general

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