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Platelets and Megakaryocytes

Volume 2: Perspectives and Techniques

  • Book
  • © 2004

Overview

Editors:
  1. Jonathan M. Gibbins

    1. School of Animal and Microbial Sciences, The University of Reading, Reading, UK

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    You can also search for this editor in PubMed   Google Scholar

  2. Martyn P. Mahaut-Smith

    1. Department of Physiology, University of Cambridge, Cambridge, UK

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    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Methods in Molecular Biology (MIMB, volume 273)

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About this book

12 The average human body has on the order of 10 circulating platelets. They are crucial for hemostasis, and yet excessive platelet activation is a major cause of m- bidity and mortality in Western societies. It is therefore not surprising that platelets have become one of the most extensively investigated biological cell types. We are, however, far from understanding precisely how platelets become activated under physiological and pathophysiological conditions. In addition, there are large gaps in our knowledge of platelet production from their giant precursor cell, the megakar- cyte. Understanding megakaryocyte biology will be crucial for the development of platelet gene targeting. The aim of Platelets and Megakaryocytes is therefore to bring together established and recently developed techniques to provide a comprehensive guide to the study of both the platelet and the megakaryocyte. It consists of five s- tions split between two volumes. The more functional assays appear in Volume 1, whereas Volume 2 includes signaling techniques, postgenomic methods, and a n- ber of key perspectives chapters. Part I of Volume 1, Platelets and Megakaryocytes: Functional Assays, describes many well-established approaches to the study of platelet function, including aggregometry, secretion, arachidonic acid metabolism, procoagulant responses, pla- let adhesion under static or flow conditions, flow cytometry, and production of microparticles. Although one would ideally wish to perform experiments with human platelets, studies within the circulation using intravital microscopy require the use of animal models, which are described in Chapter 16, vol. 1.

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Table of contents (29 protocols)

  1. Signaling Techniques

    1. Evaluation of Nitrotyrosine-Containing Proteins in Blood Platelets

      • Khalid M. Naseem, Rocio Riba, Max Troxler
      Pages 301-312

    2. Nitric Oxide Signaling in Platelets

      • Sylvia Y. Low, K. Richard Bruckdorfer
      Pages 313-333

    3. Snake Venom Toxins Affecting Platelet Function

      • Robert K. Andrews, Elizabeth E. Gardiner, Michael C. Berndt
      Pages 335-347

    4. Peptide Synthesis in the Study of Collagen-Platelet Interactions

      • C. Graham Knight, Catherine M. Onley, Richard W. Farndale
      Pages 349-363

    5. Platelet Permeabilization

      • Robert Flaumenhaft
      Pages 365-378

  2. Molecular and Post-Genomic Techniques

    1. Front Matter

      Pages 379-379
      Download chapter PDF

    2. Using Retroviruses to Express Genes in Primary Megakaryocyte Lineage Cells

      • Meenakshi Gaur, George J. Murphy, Jonathan Frampton, Andrew D. Leavitt
      Pages 381-396

    3. Use of Antisense Oligonucleotide Technology to Investigate Signaling Pathways in Megakaryocytes

      • Hava Avraham, Shalom Avraham, Radoslaw Zagozdzon
      Pages 397-406

    4. GFP Fusion Proteins to Study Signaling in Live Cells

      • Simon A. Walker, Gyles E. Cozier, Peter J. Cullen
      Pages 407-419

    5. Two-Dimensional Polyacrylamide Gel Electrophoresis for Platelet Proteomics

      • Katrin Marcus, Helmut E. Meyer
      Pages 421-433

    6. Preparation of mRNA and cDNA Libraries From Platelets and Megakaryocytes

      • Benjamin Z. S. Paul, Jiango Jin, Satya P. Kunapuli
      Pages 435-453

    7. Platelet Receptor Structures and Polymorphisms

      • Thomas J. Kunicki, Steven Head, Daniel R. Salomon
      Pages 455-478

    8. Gene Array Technology and the Study of Platelets and Megakaryocytes

      • Lloyd T. Lam, Emery H. Bresnick
      Pages 479-490

  3. Back Matter

    Pages 491-503
    Download chapter PDF
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Reviews

"...thorough and detailed...contains useful information regarding the study of platelets and megakaryocytes." - Veterinary Pathology

Editors and Affiliations

  • School of Animal and Microbial Sciences, The University of Reading, Reading, UK

    Jonathan M. Gibbins

  • Department of Physiology, University of Cambridge, Cambridge, UK

    Martyn P. Mahaut-Smith

Bibliographic Information

  • Book Title: Platelets and Megakaryocytes

  • Book Subtitle: Volume 2: Perspectives and Techniques

  • Editors: Jonathan M. Gibbins, Martyn P. Mahaut-Smith

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/1592597831

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Book Archive

  • Copyright Information: Humana Press 2004

  • Hardcover ISBN: 978-1-58829-011-3Published: 13 August 2004

  • Softcover ISBN: 978-1-61737-305-3Published: 10 November 2010

  • eBook ISBN: 978-1-59259-783-3Published: 02 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XX, 504

  • Number of Illustrations: 108 b/w illustrations, 5 illustrations in colour

  • Topics: Cell Biology

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