Overview
- Editors:
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Jonathan M. Gibbins
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School of Animal and Microbial Sciences, The University of Reading, Reading, UK
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Martyn P. Mahaut-Smith
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Department of Physiology, University of Cambridge, Cambridge, UK
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Table of contents (27 protocols)
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Platelet Functional Assays
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- Alison H. Goodall, Jackie Appleby
Pages 225-253
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- Bernhard Nieswandt, Valerie Schulte, Wolfgang Bergmeier
Pages 255-268
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- Shosaku Nomura, Shirou Fukuhara
Pages 269-277
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Megakaryocyte Functional Assays
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Front Matter
Pages 279-279
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- Najet Debili, Fawzia Louache, William Vainchenker
Pages 293-308
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- Anthony Mathur, Ying Hong, Guosu Wang, Jorge D. Erusalimsky
Pages 309-322
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- Arnaud Drouin, Gulie Alimardani, Elisabeth M. Cramer
Pages 323-345
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- Warren S. Alexander, Craig Hyland
Pages 347-359
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Back Matter
Pages 375-385
About this book
12 The average human body has in the order of 10 circulating platelets. They are crucial for hemostasis, and yet excessive platelet activation is a major cause of m- bidity and mortality in western societies. It is therefore not surprising that platelets have become one of the most extensively investigated biological cell types. We are, however, far from understanding precisely how platelets become activated under physiological and pathophysiological conditions. In addition, there are large gaps in our knowledge of platelet production from their giant precursor cell, the megakar- cyte. Understanding megakaryocyte biology will be crucial for the development of platelet gene targeting. The aim of Platelets and Megakaryocytes is therefore to bring together established and recently developed techniques to provide a comprehensive guide to the study of both the platelet and the megakaryocyte. It consists of five s- tions split between two volumes. The more functional assays appear in Volume 1, whereas Volume 2 includes signaling techniques, postgenomic methods, and a n- ber of key perspectives chapters. Part I of Volume 1, Platelets and Megakaryocytes: Functional Assays, describes many well established approaches to the study of platelet function, including aggregometry, secretion, arachidonic acid metabolism, procoagulant responses, pla- let adhesion under static or flow conditions, flow cytometry, and production of microparticles. Although one would ideally wish to perform experiments with human platelets, studies within the circulation using intravital microscopy require the use of animal models, which are described in Chapter 16, vol. 1.
Reviews
"...thorough and detailed...contains useful information regarding the study of platelets and megakaryocytes." - Veterinary Pathology
Editors and Affiliations
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School of Animal and Microbial Sciences, The University of Reading, Reading, UK
Jonathan M. Gibbins
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Department of Physiology, University of Cambridge, Cambridge, UK
Martyn P. Mahaut-Smith