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Gene Probes

Principles and Protocols

  • Book
  • © 2002

Overview

Editors:
  1. Marilena Aquino Muro

    1. CABI Bioscience UK Centre, Surrey, UK

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    You can also search for this editor in PubMed   Google Scholar

  2. Ralph Rapley

    1. University of Hertfordshire, UK

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    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Methods in Molecular Biology (MIMB, volume 179)

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About this book

Senior scientists Marilena Aquino de Muro and Ralph Rapley have brought together an outstanding collection of time-tested protocols for designing and using genes probes in a wide variety of applications. The applications covered range from searching for specific genes in the human chromosome to the detection of microorganisms and their toxins in the environment and in food samples, as well as in the diagnosis of human disease. Helpful tutorials explain the principles of gene probe design, labeling, detection, target formation, and hybridization conditions.

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Table of contents (23 protocols)

  1. Front Matter

    Pages i-x
    Download chapter PDF

  2. Target Format and Hybridization Conditions

    • Alex Reid
    Pages 1-11

  3. End Labeling Procedures

    • Elena Hilario
    Pages 13-18

  4. Photobiotin Labeling

    • Elena Hilario
    Pages 19-22

  5. Nick Translation

    • Alex Reid
    Pages 23-25

  6. Random Primed Labeling

    • Alex Reid
    Pages 27-28

  7. Design and Evaluation of 16S rRNA-Targeted Oligonucleotide Probes for Fluorescence In Situ Hybridization

    • Philip Hugenholtz, Gene W. Tyson, Linda L. Blackall
    Pages 29-42

  8. Evaluation of Alterations in the Tumor Suppressor Genes INK4A and INK4B in Human Bladder Tumors

    • Irene Orlow, Carlos Cordon-Cardo
    Pages 43-59

  9. The β-Tubulin Gene Region as a Molecular Marker to Distinguish Leishmania Parasites

    • Alexis Mendoza-León, Luis Luis, Clara Martínez
    Pages 61-83

  10. Detection of Malignant Plasma Cells in the Bone Marrow and Peripheral Blood of Patients with Multiple Myeloma

    • Ross D. Brown, P. Joy Ho
    Pages 85-91

  11. Detection of Chlamydia trachomatis by DNA Amplification

    • Peter Timms, Sarah Mathews
    Pages 93-100

  12. Isolation and Identification of Campylobacter Species by Use of Selective Enrichment, Nucleic Acid Amplification Methods, and Gene Probes

    • Alex van Belkum, Nicole van den Braak, Leen-Jan van Doorn, Hubert Endtz
    Pages 101-111

  13. Diagnosis of Human Papillomavirus Using In Situ Hybridization and In Situ Polymerase Chain Reaction

    • Gerard J. Nuovo
    Pages 113-136

  14. Quantitation of In Situ Hybridization Using Image Analysis of Radioactively Labeled RNA Probes

    • Teresa Bisucci, Tim D. Hewitson, Ian A. Darby
    Pages 137-147

  15. Stable Isotope Probing for Detection of Methanotrophs After Enrichment with 13CH4

    • Stefan Radajewski, J. Colin Murrell
    Pages 149-157

  16. Detection of Enterococci in Freshwater and Seawater (16S and 23S rRNA Enterococcus Oligonucleotide Probes)

    • Gillian D. Lewis, Sally A. Anderson, Susan J. Turner
    Pages 159-169

  17. Analysis of 16S–23S rRNA Intergenic Spacer of Vibrio cholerae and Vibrio mimicus for Detection of These Species

    • Jongsik Chun, Irma N. G. Rivera, Rita R. Colwell
    Pages 171-178

  18. Colony Hybridization of Bacterial Isolates with Burkholderia cepacia-Specific Probes

    • Laura G. Leff
    Pages 179-185

  19. In Situ Hybridization of Burkholderia cepacia Cells

    • Christopher J. McNamara, Laura G. Leff
    Pages 187-192

  20. Detection of Polychlorinated Biphenyl-Degrading Organisms in Soil

    • John Sanseverino, Alice C. Layton, Gary S. Sayler
    Pages 193-209
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Reviews

"In this book, the editors have brought together a comprehensive collection of time-tested protocols for designing and using gene probes in a wide variety of applications." -Clinical Laboratory International

Editors and Affiliations

  • CABI Bioscience UK Centre, Surrey, UK

    Marilena Aquino Muro

  • University of Hertfordshire, UK

    Ralph Rapley

Bibliographic Information

  • Book Title: Gene Probes

  • Book Subtitle: Principles and Protocols

  • Editors: Marilena Aquino Muro, Ralph Rapley

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/1592592384

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Book Archive

  • Copyright Information: Humana Press 2002

  • Hardcover ISBN: 978-0-89603-885-1Published: 20 September 2001

  • Softcover ISBN: 978-1-61737-236-0Published: 10 November 2010

  • eBook ISBN: 978-1-59259-238-8Published: 03 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 272

  • Topics: Biochemistry, general

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