Overview
- Editors:
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Stephen P. Robinson
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Department of Hematology, University College Hospital London, UK
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Andrew J. Stagg
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Antigen Presentation Research Group, Imperial College School of Medicine, at Northwick Park Hospital, Harrow, UK
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Stella C. Knight
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Professor of Immunopathology, Imperial College School of Medicine, London
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Table of contents (38 protocols)
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Derivation of Animal Dendritic Cells from Progenitor Cells
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- Timothy Powell, James R. Major, Gordon MacPherson
Pages 199-205
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- Karen Lucas, Dolores Saunders, Li Wu
Pages 207-216
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Generation of Murine Dendritic Cell Lines
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Front Matter
Pages 217-217
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- Stefania Citterio, Maria Rescigno, Maria Foti, Francesca Granucci, Malgosia Matyszak, Giampiero Girolomoni et al.
Pages 219-230
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- Thilo Jakob, Mark C. Udey
Pages 231-241
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- Akira Takashima, Paul R. Bergstresser
Pages 243-254
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Generation of Human Dendritic Cells from Progenitor Cells
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Front Matter
Pages 255-255
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- Christophe Caux, Bertrand Dubois
Pages 257-273
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- Winfried F. Pickl, Otto Majdic, Walter Knapp
Pages 283-296
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- Michel F. Gilliet, Frank O. Nestle
Pages 297-303
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Aspects of Dendritic Cell Function
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Front Matter
Pages 305-305
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- Walter Luini, Giovanna D’Amico, Sergio Bernasconi, Paola Allavena, Alberto Mantovani, Silvano Sozzani
Pages 307-312
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- Giancarlo Bianchi, Giovanna D’Amico, Silvano Sozzani, Alberto Mantovani, Paola Allavena
Pages 325-330
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- Marie Cumberbatch, Rebecca J. Dearman, Ian Kimber
Pages 331-346
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- Kenjiro Matsuno, Taichi Ezaki
Pages 347-368
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- Christophe Caux, Bertrand Dubois
Pages 369-376
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- Neil A. Fanger, Paul M. Guyre, Robert F. Graziano
Pages 377-386
About this book
Dendritic Cell Protocols provides chapter and verse for many useful practical approaches to the art of studying dendritic cells. The book gives information on the usual techniques for derivation of human dendritic cells from precursor stem cells, such as monocytes. In addition it provides data on the difficult tasks of isolating dendritic cells directly from different tissues; whether dendritic cells from precursor cells or from tissues of mouse or human are required, this book contributes practical information. The last section of the book is devoted to functional aspects of dendritic cells ranging from inf- mation relevant to cell migration to antigen uptake and T cell stimulation. But what is a dendritic cell? The explosion of studies on this cell type over the last few years is breathtaking. We now have so-called “myeloid” and “plas- cytoid” dendritic cells; are they of separate lineages or are they related? There are claims that dendritic cells can be derived from B cells and from granulocytes, as well as from “monocytes. ” Lymphocytes can be switched on or off by dendritic cells. Dendritic cells can preferentially cause Th1 or Th2 responses or some may stimulate B cells directly. When there is so much scope for exploring these new developments, it is particularly important that the data that are established and the practical basis for this work are clearly available, a task that this book fulfils.
Reviews
". . .the purposes of the book are worthwhile and are well attained. . .The book could be used effectively by experienced or beginning researchers in the field since the protocols are written clearly and concisely. There are 38 brief chapters in this book by over 70 authors, who are among the most experienced and knowledgeable researchers in the field. . .Among the many experimental protocols described in this book are methods for isolating, culturing, and establishing cell lines from many organs from murine and human sources. The protocols are written clearly and should serve as a useful laboratory resource for anyone wishing to learn how to study these cells involved in the first line of defense of the immune system. The book has a utilitarian look and feel. . .It would be quite useful for anyone doing bench research on murine or human dendritic cells."-Doody's Health Sciences Book Review Journal
"The layout of the protocols is in a step-by-step fashion which makes them relatively easy to follow. Moreover, they contain enough of the fine details that are so often disregarded in published scientific manuscripts, thus ensuring their reproducibility. Hence, Dendritic Cell Protocols will prove to be an extremely useful source of practical information for novice DC researchers." - Immunology News
Editors and Affiliations
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Department of Hematology, University College Hospital London, UK
Stephen P. Robinson
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Antigen Presentation Research Group, Imperial College School of Medicine, at Northwick Park Hospital, Harrow, UK
Andrew J. Stagg
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Professor of Immunopathology, Imperial College School of Medicine, London
Stella C. Knight