Overview
- Editors:
-
-
Anthony P. Corfield
-
Bristol Royal Infirmary, Bristol, UK
Access this book
Other ways to access
Table of contents (41 protocols)
-
Carbohydrate Structural Analysis of Mucins
-
- Jean-Claude Michalski, Calliope Capon
Pages 159-180
-
-
- André Klein, Gérard Strecker, Geneviève Lamblin, Philippe Roussel
Pages 191-209
-
- Mathew J. Harrison, Nicolle H. Packer
Pages 211-216
-
Mucin Biosynthesis
-
Front Matter
Pages 217-217
-
- Anthony P. Corfield, Neil Myerscough, Alexandra W. C. Einerhand, B. Van Jan-Willem Klinken, Jan Dekker, Christos Paraskeva
Pages 219-226
-
- Anthony P. Corfield, Neil Myerscough, B. Van Jan-Willem Klinken, Alexandra W. C. Einerhand, Jan Dekker
Pages 227-237
-
- B. Van Jan-Willem Klinken, Hans A. Büller, Alexandra W. C. Einerhand, Jan Dekker
Pages 239-247
-
- Alexandra W. C. Einerhand, B. Van Jan-Willem Klinken, Hans A. Buller, Jan Dekker
Pages 249-259
-
- Guillemette Huet, Philippe Delannoy, Thécla Lesuffleur, Sylviane Hennebicq, Pierre Degand
Pages 261-271
-
-
Mucin Gene Detection
-
Front Matter
Pages 295-295
-
- Jean-Pierre Aubert, Nicole Porchet
Pages 297-303
-
- Nicole Porchet, Jean-Pierre Aubert
Pages 305-312
-
- Nicole Porchet, Jean-Pierre Aubert
Pages 313-321
-
-
- Lynne E. Vinall, Wendy S. Pratt, Dallas M. Swallow
Pages 337-350
-
Preparation of Antimucin Antibodies
-
Front Matter
Pages 351-351
-
- Francisco X. Real, Carme de Bolós, Egbert Oosterwijk
Pages 353-368
-
- Pei Xiang Xing, Vasso Apostolopoulos, Jim Karkaloutsos, Ian F. C. McKenzie
Pages 369-381
About this book
The mucins (mucus glycoproteins) have long been a complex corner of glycoprotein biology. While dramatic advances in the separation, structural an- ysis, biosynthesis, and degradation have marked the progress in general glycop- tein understanding, the mucins have lagged behind. The reasons for this lack of progress have always been clear and are only now being resolved. The mucins are very large molecules; they are difficult to separate from other molecules present in mucosal secretions or membranes; they are often degraded owing to natural protective functions or to isolation methodology and their peptide and oligos- charide structures are varied and complex. Understanding these molecules has demanded progress in several major areas. Isolation techniques that protect the intact mucins and allow dissociation from other adsorbed but discrete molecules needed to be developed and accepted by all researchers in the field. Improved methods for the study of very large molecules with regard to their aggregation and polymerization were also needed. Structural analysis of the peptide domains and the multitude of oligosaccharide chains was required for smaller sample sizes, for multiple samples, and in shorter time. In view of these problems it is perhaps not surprising that the mucins have remained a dilemma, of obvious biological importance and interest, but very difficult to analyze.
Reviews
"There is sufficient detail to follow easily and unlike many research papers, the minute details which make for success of failure, all appear to included." -Microbiology Today
Editors and Affiliations
-
Bristol Royal Infirmary, Bristol, UK
Anthony P. Corfield