Overview
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 125)
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About this book
The mucins (mucus glycoproteins) have long been a complex corner of glycoprotein biology. While dramatic advances in the separation, structural an- ysis, biosynthesis, and degradation have marked the progress in general glycop- tein understanding, the mucins have lagged behind. The reasons for this lack of progress have always been clear and are only now being resolved. The mucins are very large molecules; they are difficult to separate from other molecules present in mucosal secretions or membranes; they are often degraded owing to natural protective functions or to isolation methodology and their peptide and oligos- charide structures are varied and complex. Understanding these molecules has demanded progress in several major areas. Isolation techniques that protect the intact mucins and allow dissociation from other adsorbed but discrete molecules needed to be developed and accepted by all researchers in the field. Improved methods for the study of very large molecules with regard to their aggregation and polymerization were also needed. Structural analysis of the peptide domains and the multitude of oligosaccharide chains was required for smaller sample sizes, for multiple samples, and in shorter time. In view of these problems it is perhaps not surprising that the mucins have remained a dilemma, of obvious biological importance and interest, but very difficult to analyze.
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Table of contents (41 protocols)
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Front Matter
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Purification of Mucin
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Front Matter
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Detection and Ouantitation of Mucin
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Front Matter
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Separation, Identification, and Physical Characterization of Mucins
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Front Matter
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Rheology of Mucin
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Front Matter
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Carbohydrate Structural Analysis of Mucins
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Front Matter
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Reviews
"There is sufficient detail to follow easily and unlike many research papers, the minute details which make for success of failure, all appear to included." -Microbiology Today
Editors and Affiliations
Bibliographic Information
Book Title: Glycoprotein Methods and Protocols
Book Subtitle: The Mucins
Editors: Anthony P. Corfield
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1385/1592590489
Publisher: Humana Totowa, NJ
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eBook Packages: Springer Book Archive
Copyright Information: Humana Press 2000
Hardcover ISBN: 978-0-89603-720-5Published: 01 March 2000
Softcover ISBN: 978-1-61737-149-3Published: 10 November 2010
eBook ISBN: 978-1-59259-048-3Published: 26 October 2007
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XVII, 506
Topics: Biochemistry, general