Overview
- Editors:
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Y. M. Dennis Lo
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The Chinese University of Hong Kong, Hong Kong SAR
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Table of contents (26 protocols)
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Clinical Application
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- Akihiko Sekizawa, Hiroshi Saito, Takumi Yanaihara
Pages 275-285
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- Barbara Pertl, Matteo Adinolfi
Pages 287-299
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- Christopher John Healey, Steven Read
Pages 301-307
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- Majella Maher, Maura Glennon, Martin Cormican, Terry Smith
Pages 309-319
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- John Koreth, Christopher J., James O′D. McGee
Pages 321-339
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Back Matter
Pages 341-353
About this book
The polymerase chain reaction (PCR) is one of the most important molecular biological methods ever devised, with numerous applications to cli- cal molecular medicine. Since its description in 1985, PCR has undergone tremendous improvements, and many variations on the basic PCR theme have been published. With such a large volume of PCR-related literature, a clinical scientist wishing to use the technique will have a difficult task loc- ing the relevant information to implement it effectively. There is thus clearly a need for an up-to-date volume with detailed protocols to facilitate the setting up of those techniques most relevant to clinical applications. Unlike some other books on this topic, Clinical Applications of PCR includes only methods that are of direct relevance in clinical settings. The book is organized in three parts: an introductory section, a section on general methodology, and a final section with specific clinical applications. The first section covers the basic principles of PCR and is most useful to those new to molecular diagnosis. The next chapter includes useful tips for setting up a PCR laboratory. Section 2 then outlines some of the most commonly used PCR-based techniques in molecular diagnosis. Section 3 includes carefully chosen examples that represent typical applications of PCR in diverse clinical fields, encompassing hematology, oncology, genetics, and microbiology.
Reviews
". . . one of the first texts to carefully document all the needs required to perform PCR. . . [By] covering the fundamentals, methodologies, and applications of PCR, the editor has created a unique document that will be welcome in the clinical laboratory."-Doody's Health Sciences Book Review Journal
"Each chapter is clearly written and provides enough background material to explain the significance and application of the procedure. The protocols include sufficient detail to enable the reader to develop the test. This manual will be an important laboratory resource to those using PCR in the diagnosis of human diseases and should be of great interest to both molecular and clinical pathologists."-Archives of Pathology and Laboratory Medicine
Editors and Affiliations
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The Chinese University of Hong Kong, Hong Kong SAR
Y. M. Dennis Lo