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Human Cell Culture Protocols

  • Book
  • © 1996

Overview

Editors:
  1. Gareth E. Jones

    1. King’s College, University of London, UK

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Part of the book series: Methods in Molecular Medicine (MIMM, volume 2)

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Table of contents (41 protocols)

  1. Isolation and Culture of Bone-Forming Cells (Osteoblasts) from Human Bone

    • James A. Gallagher, Roger Gundle, Jon N. Beresford
    Pages 233-262

  2. The Isolation of Osteoclasts from Human Giant Cell Tumors and Long-Term Marrow Cultures

    • Catherine A. Walsh, John A. Carron, James A. Gallagher
    Pages 263-276

  3. In Vitro Cellular Systems for Studying OC Function and Differentiation

    • Donatella Aldinucci, Julian M. W. Quinn, Massimo Degan, Senka Juzbasic, Angela De Luliis, Salvatore Improta et al.
    Pages 277-306

  4. Isolation, Purification, and Growth of Human Skeletal Muscle Cells

    • Grace K. Pavlath
    Pages 307-317

  5. Cultures of Proliferating Vascular Smooth Muscle Cells from Adult Human Aorta

    • Heide L. Kirschenlohr, James C. Metcalfe, David J. Grainger
    Pages 319-334

  6. Human Myometrial Smooth Muscle Cells and Cervical Fibroblasts in Culture

    • Françoise Cavaillé, Dominique Cabrol, Françoise Ferre
    Pages 335-344

  7. Primary Culture of Human Antral Endocrine Cells

    • Alison M. J. Buchan
    Pages 345-355

  8. Primary Cell Culture of Human Enteric Neurons

    • Eric A. Accili, Alison M. J. Buchan
    Pages 357-367

  9. Isolation and Culture of Human Hepatocytes

    • Martin K. Bayliss, Paul Skett
    Pages 369-389

  10. Culture of Human Pancreatic Islet Cells

    • Stellan Sandle, Décio L. Eizirik
    Pages 391-407

  11. Isolation and Culture of Human Renal Cortical Cells with Characteristics of Proximal Tubules

    • Vicente Rodilla, Gabrielle M. Hawksworth
    Pages 409-417

  12. Glomerular Epithelial and Mesangial Cell Culture and Characterization

    • Heather M. Wilson, Keith N. Stewart, Alison M. MacLeod
    Pages 419-430

  13. Enzymatic Isolation and Serum-Free Culture of Human Renal Cells

    • John H. Todd, Kenneth E. McMartin, Donald A. Sens
    Pages 431-435

  14. Culture of Human Renal Medullary Interstitial Cells

    • Klara Tisocki, Gabrielle M. Hawksworth
    Pages 437-445

  15. Culture Technique of Human Pituitary Adenoma Cells

    • Masanori Kabuto
    Pages 447-455

  16. Preparation of Human Trophoblast Cells for Culture

    • Suren R. Sooranna
    Pages 457-464

  17. Superfused Microcarrier Cultures of BeWo Choriocarcinoma Cells

    • Suren R Sooranna, Bryan M. Eaton
    Pages 465-476

  18. Cornea1 Organ Culture

    • Robert W. Lambert, Normand R. Richard, Janet A. Anderson
    Pages 477-488

  19. Keratocyte Cell Culture

    • Normand R. Richard, Robert W. Lambert, Janet A. Anderson
    Pages 489-501

  20. Conjunctiva

    • Monica Berry, Roger B. Ellingham, Anthony P. Corfield
    Pages 503-516
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About this book

Expert scientific and clinical investigators present proven human cell culture techniques applicable to tissue samples taken from a wide variety of organs, particularly those prone to pathological change. They describe in clear, step-by-step instructions the special requirements for successfully culturing such human cells as T-cells, trophoblast cells, renal cells, natural killer cells, endothelial cells, neurons, epithelial cells, pituitary cells, and more. The protocols eliminate much of the chore of adapting techniques initially developed for animal cell culture systems or the time spent in hunting down potentially useful techniques buried in the details of research papers, or even in books largely devoted to animal cell culture. They allow researchers to use successfully high quality in vitro cultures as models to explore the disease process.

Reviews

This book is for the clinical scientist beginning to use in vitro systems and for anyone making the transition from animal cell culture to the use of human tissue and cell lines.- Journal of Toxicology: Clinical Toxicology

Editors and Affiliations

  • King’s College, University of London, UK

    Gareth E. Jones

Bibliographic Information

  • Book Title: Human Cell Culture Protocols

  • Editors: Gareth E. Jones

  • Series Title: Methods in Molecular Medicine

  • DOI: https://doi.org/10.1385/089603335X

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 1996

  • eBook ISBN: 978-1-59259-586-0Published: 02 February 2008

  • Series ISSN: 1543-1894

  • Series E-ISSN: 1940-6037

  • Edition Number: 1

  • Number of Pages: XIV, 545

  • Number of Illustrations: 101 b/w illustrations

  • Topics: Cell Biology

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