Skip to main content
SpringerLink
Log in

New Nucleic Acid Techniques

  • Book
  • © 1988

Overview

Editors:
  1. John M. Walker

    1. The Hatfield Polytechnic, Hatfield, UK

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Methods in Molecular Biology (MIMB, volume 4)

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 169.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 219.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

Table of contents (44 protocols)

  1. Immunoscreening of λgt11 Expression Libraries Using an Avidin–Biotin Detection System

    • Robert McGookin
    Pages 301-305

  2. Primed Synthesis and Direct Sequencing in the Isolation of cDNA Clones Using Short Oligonucleotides

    • John D. Haley
    Pages 307-318

  3. Constructing Expression cDNA Libraries Using Unphosphorylated Adaptors

    • Keith K. Stanley, Joachim Herz, Harald Haymerle
    Pages 319-328

  4. Expression Screening of cDNA Libraries in pEX

    • Keith K. Stanley
    Pages 329-342

  5. Producing Antibodies of Predetermined Specificity from Escherichia coli Hybrid Proteins

    • Keith K. Stanley
    Pages 343-349

  6. Epitope Mapping Using pEX

    • Keith K. Stanley
    Pages 351-361

  7. DNA Transfection of Mammalian Cells Using Polybrene

    • William G. Chaney, Daniel R. Howard, Jeffrey W. Pollard, Sandra Sallustio, Pamela Stanley
    Pages 363-370

  8. Expression of Foreign Genes in Mammalian Cells

    • Martin J. Page
    Pages 371-384

  9. Dot-Blot Hybridization Method

    • Hans E. W. Bergmans, Wim Gaastra
    Pages 385-390

  10. Detection of Unique or Low Copy Number DNA Sequences in Complex Genomes

    • Domingo Gallardo, Albert Boronat
    Pages 391-399

  11. Photobiotin-Labeled DNA and RNA Hybridization Probes

    • James L. McInnes, Anthony C. Forster, Robert H. Symons
    Pages 401-414

  12. Enzyme-Labeled DNA Probes

    • J. Lesley Woodhead, Rachel Fallon, Hermia Figueiredo, Alan D. B. Malcolm
    Pages 415-420

  13. Luminescent Detection of Specific DNA Sequences

    • J. Lesley Woodhead, Hermia Figueiredo, Alan D. B. Malcolm
    Pages 421-424

  14. Detection of DNA Sequences Using Biotinylated Probes

    • J. Lesley Woodhead, Hermina Figueiredo, Alan D. B. Malcolm
    Pages 425-430

  15. Crosslinking of Single-Stranded DNA to Resins

    • J. Lesley Woodhead, Jane A. Langdale, Alan D. B. Malcolm
    Pages 431-436

  16. Extraction of RNA from Plants

    • Adrian Slater
    Pages 437-446

  17. Isolation of Plant Nuclei

    • Richard D. Henfrey, Robert J. Slater
    Pages 447-452

  18. In Vitro Transcription in Plant Nuclei

    • Richard D. Henfrey, Robert J. Slater
    Pages 453-463

  19. Large-Scale Isolation of Ti Plasmid DNA

    • Kevan M. A. Gartland
    Pages 465-470

  20. Transformation and Regeneration of Dicotyledonous Plants

    • Kevan M. A. Gartland, Nigel W. Scott
    Pages 471-479
Back to top

Editors and Affiliations

  • The Hatfield Polytechnic, Hatfield, UK

    John M. Walker

Bibliographic Information

  • Book Title: New Nucleic Acid Techniques

  • Editors: John M. Walker

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/0896031276

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 1988

  • Hardcover ISBN: 978-0-89603-127-2Published: 31 August 1988

  • Softcover ISBN: 978-1-4899-4383-5Published: 22 August 2013

  • eBook ISBN: 978-1-59259-491-7Published: 13 May 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XVI, 560

  • Number of Illustrations: 17 b/w illustrations

  • Topics: Biochemistry, general

Publish with us

Policies and ethics

Back to top

Search

Navigation