Overview
- Editors:
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Michail A. Alterman
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Office of Policy for Pharmaceutical Quality Office of Pharmaceutical Quality, Center for Drug Evaluation and Research US FDA, Silver Spring, USA
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
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Table of contents (33 protocols)
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- MarÃa Castro-Puyana, MarÃa Luisa Marina
Pages 277-291
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- Akiyoshi Hirayama, Satsuki Ikeda, Asako Sato, Tomoyoshi Soga
Pages 307-313
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- Gordana D. Žunić, Slavica Spasić, Zorana Jelić-Ivanović
Pages 315-326
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- Meera Shanmuganathan, Philip Britz-McKibbin
Pages 327-350
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- Marilda Rigobello-Masini, Jorge C. Masini
Pages 351-364
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- Jörg Ziegler, Hina Hussain, Reinard H. H. Neubert, Steffen Abel
Pages 365-379
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- Ine Rombouts, Bert Lagrain, Lieve Lamberts, Inge Celus, Kristof Brijs, Jan A. Delcour
Pages 381-394
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- Madeleine Werneth, Gunda Koellensperger, Stephan Hann
Pages 395-402
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- Björn Thiele, Michelle Hupert, Beatrix Santiago-Schübel, Marco Oldiges, Diana Hofmann
Pages 403-414
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- A. Pappa-Louisi, P. Agrafiotou, S. Sotiropoulos
Pages 415-428
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- Ward van Helmond, Vincent O’Brien, Robin de Jong, Jan van Esch, Sander Oldenhof, Marcel de Puit
Pages 429-438
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- Ward van Helmond, Marcel de Puit
Pages 439-450
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Back Matter
Pages 451-455
About this book
Amino Acid Analysis (AAA) is an integral part of analytical biochemistry. In a relatively short time, the variety of AAA methods has evolved dramatically with more methods shifting to the use of mass spectrometry (MS) as a detection method; another new aspect is miniaturization. However, most importantly, AAA in this day and age should be viewed in the context of Metabolomics as a part of Systems Biology. Amino Acid Analysis: Methods and Protocols, Second Edition presents a broad spectrum of all available methods allowing readers to choose the method that best suits their particular laboratory set-up and analytical needs. In this volume, readers can find chapters describing general, as well as specific approaches to the sample preparation. A number of chapters describe specific applications of AAA in clinical chemistry, as well as in food analysis, microbiology, marine biology, drug metabolism, even archaeology. Separate chapters are devoted to the application of AAA for protein quantitation and chiral AAA. Written in the highly successful Methods in Molecular Biology series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and accessible, Amino Acid Analysis: Methods and Protocols, Second Edition provides crucial techniques that can be applied across multiple disciplines by anyone involved in biomedical research or life sciences.
Editors and Affiliations
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Office of Policy for Pharmaceutical Quality Office of Pharmaceutical Quality, Center for Drug Evaluation and Research US FDA, Silver Spring, USA
Michail A. Alterman