Skip to main content
SpringerLink
Log in

Beta-Arrestins

Methods and Protocols

  • Book
  • © 2019

Overview

Editors:
  1. Mark G. H. Scott

    1. Institut Cochin, INSERM U1016, Paris, France

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Stéphane A. Laporte

    1. Department of Medicine, Research Institute of the McGill, University Health Center (RI-MUHC), McGill University, Montreal, Canada

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1957)

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 129.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

Table of contents (25 protocols)

  1. Methods to Study β-Arrestin Localization

    1. Front Matter

      Pages 249-249
      Download chapter PDF

    2. Methods to Investigate the Nucleocytoplasmic Shuttling Properties of β-Arrestins

      • Elodie Blondel-Tepaz, Thomas Guilbert, Mark G. H. Scott
      Pages 251-269

    3. Monitoring β-Arrestin 2 Targeting to the Centrosome, Basal Body, and Primary Cilium by Fluorescence Microscopy

      • Anahi Molla-Herman, Kathryn M. Davis, Kirk Mykytyn, Alexandre Benmerah
      Pages 271-289

  2. Methods to Study Conformational Change of β-Arrestin

    1. Front Matter

      Pages 291-291
      Download chapter PDF

    2. A Mass Spectrometry-Based Structural Assay for Activation-Dependent Conformational Changes in β-Arrestins

      • Yang Zhao, Kunhong Xiao
      Pages 293-308

    3. Probing Arrestin Function Using Intramolecular FlAsH-BRET Biosensors

      • Erik G. Strungs, Louis M. Luttrell, Mi-Hye Lee
      Pages 309-322

  3. Methods to Study Roles of β-Arrestins in Physiopathology

    1. Front Matter

      Pages 323-323
      Download chapter PDF

    2. Methods to Study the Roles of β-Arrestins in Meningococcal Signaling

      • Zoé Virion, Stefano Marullo, Mathieu Coureuil
      Pages 325-334

    3. Methods to Investigate the Roles for β-Arrestin-2 in Allergic Inflammatory Airway Disease

      • Akhil Hegde, Julia K. L. Walker
      Pages 335-343

    4. Methods to Study Roles of β-Arrestins in the Regulation of Pancreatic β-Cell Function

      • Stéphane Dalle, Safia Costes, Gyslaine Bertrand, Magalie A. Ravier
      Pages 345-364

    5. Methods to Investigate β-Arrestin Function in Metabolic Regulation

      • Bing Luan, Jian Zhao, Gang Pei
      Pages 365-384

    6. Methods to Investigate the Role of β-Arrestin Signaling in Parkinson’s Disease

      • Nikhil M. Urs
      Pages 385-391

    7. Methods to Investigate β-Arrestin-1/β-Catenin Signaling in Ovarian Cancer Cells

      • Laura Rosanò, Roberta Cianfrocca, Anna Bagnato
      Pages 393-406

  4. Back Matter

    Pages 407-409
    Download chapter PDF
Back to top

Keywords

About this book

This volume looks at various approaches to study the pleiotropic roles of b-arrestins (b-arrs) in the control of signal transduction, and the resulting cellular and in vivo consequences that arise. The chapters in this book cover diverse topics around b-arrs such as their established roles in GPCR regulation and trafficking; regulatory scaffolding functions of b-arrs in  MAPK signaling, cAMP hydrolysis and cytoskeletal dynamics; proteomic analysis of the b-arr interactome; mathematical modelling of b-arr signaling networks; functional selectivity involving biased ligands; nucleocytoplasmic trafficking and primary cilia-associated functions of b-arrs; conformational plasticity of b-arrs; and the roles of b-arrs in allergic inflammation, Type 2 Diabetes, Parkinson’s disease, and cancer. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents,step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Cutting-edge and authoritative, Beta-Arrestins: Methods and Protocols is a valuable resource for researchers interested in learning more about the function and regulation of b-arrestins.


Editors and Affiliations

  • Institut Cochin, INSERM U1016, Paris, France

    Mark G. H. Scott

  • Department of Medicine, Research Institute of the McGill, University Health Center (RI-MUHC), McGill University, Montreal, Canada

    Stéphane A. Laporte

Bibliographic Information

  • Book Title: Beta-Arrestins

  • Book Subtitle: Methods and Protocols

  • Editors: Mark G. H. Scott, Stéphane A. Laporte

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9158-7

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-9157-0Published: 28 March 2019

  • eBook ISBN: 978-1-4939-9158-7Published: 27 March 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 409

  • Number of Illustrations: 19 b/w illustrations, 60 illustrations in colour

  • Topics: Protein Science, Cell Biology, Receptors

Publish with us

Policies and ethics

Back to top

Search

Navigation