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Bioluminescence

Methods and Protocols

  • Book
  • © 2016

Overview

Editors:
  1. Sung Bae Kim

    1. Natl Inst of AIST Res Inst for Envt Mgt Tech, Tsukuba, Japan

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1461)

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Table of contents (25 protocols)

  1. Front Matter

    Pages i-xiii
    Download chapter PDF

  2. Ingredients of Bioluminescent Probes

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Label-Free Cell Phenotypic Identification of d-Luciferin as an Agonist for GPR35

      • Heidi Hu, Huayun Deng, Ye Fang
      Pages 3-17

    3. Synthetic Bioluminescent Coelenterazine Derivatives

      • Ryo Nishihara, Daniel Citterio, Koji Suzuki
      Pages 19-31

    4. Molecular Cloning of Secreted Luciferases from Marine Planktonic Copepods

      • Yasuhiro Takenaka, Kazuho Ikeo, Yasushi Shigeri
      Pages 33-41

    5. How to Fabricate Functional Artificial Luciferases for Bioassays

      • Sung-Bae Kim, Rika Fujii
      Pages 43-53

    6. Quantum Yield Determination Based on Photon Number Measurement, Protocols for Firefly Bioluminescence Reactions

      • Kazuki Niwa
      Pages 55-61

  3. Fabrication of Bioluminescent Probes

    1. Front Matter

      Pages 63-63
      Download chapter PDF

    2. Bioluminescent Ligand–Receptor Binding Assays for Protein or Peptide Hormones

      • Ya-Li Liu, Zhan-Yun Guo
      Pages 65-90

    3. Bioluminogenic Imaging of AminopeptidaseN In Vitro and In Vivo

      • Wenxiao Wu, Laizhong Chen, Jing Li, Lupei Du, Minyong Li
      Pages 91-99

    4. Firefly Luciferase-Based Sequential Bioluminescence Resonance Energy Transfer (BRET)-Fluorescence Resonance Energy Transfer (FRET) Protease Assays

      • Bruce Branchini
      Pages 101-115

    5. Monitoring Intracellular pH Change with a Genetically Encoded and Ratiometric Luminescence Sensor in Yeast and Mammalian Cells

      • Yunfei Zhang, J. Brian Robertson, Qiguang Xie, Carl Hirschie Johnson
      Pages 117-130

    6. A Protein–Protein Interaction Assay FlimPIA Based on the Functional Complementation of Mutant Firefly Luciferases

      • Yuki Ohmuro-Matsuyama, Hiroshi Ueda
      Pages 131-142

    7. Single-Chain Probes for Illuminating Androgenicity of Chemicals

      • Sung-Bae Kim, Hiroaki Tao
      Pages 143-151

    8. Multicolor Imaging of Bifacial Activities of Estrogens

      • Sung-Bae Kim, Yoshio Umezawa
      Pages 153-163

    9. Circular Permutation Probes for Illuminating Phosphorylation of Estrogen Receptor

      • Sung-Bae Kim, Hiroaki Tao
      Pages 165-173

    10. Fabrication of Molecular Strain Probes for Illuminating Protein–Protein Interactions

      • Sung-Bae Kim, Rika Fujii
      Pages 175-182

    11. An ALuc-Based Molecular Tension Probe for Sensing Intramolecular Protein–Protein Interactions

      • Sung-Bae Kim, Ryo Nishihara, Koji Suzuki
      Pages 183-193

    12. Live Cell Bioluminescence Imaging in Temporal Reaction of G Protein-Coupled Receptor for High-Throughput Screening and Analysis

      • Mitsuru Hattori, Takeaki Ozawa
      Pages 195-202

    13. Imaging Histone Methylations in Living Animals

      • Thillai V. Sekar, Ramasamy Paulmurugan
      Pages 203-215
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Keywords

About this book

This volume presents detailed laboratory protocols regarding the three major route technologies luciferases, efficient optical probes, and applications to visualizing molecular events in living subjects. Bioluminescence: Methods and Protocols, Third Edition guides readers through chapters on ingredients of bioluminescent probes, and fabrication of bioluminescent probes, applications to living subjects and instrumentations. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Bioluminescence: Methods and Protocols, Third Edition is a useful complement to the first and second edition for new and experienced researchers alike.

 

Editors and Affiliations

  • Natl Inst of AIST Res Inst for Envt Mgt Tech, Tsukuba, Japan

    Sung Bae Kim

Bibliographic Information

  • Book Title: Bioluminescence

  • Book Subtitle: Methods and Protocols

  • Editors: Sung Bae Kim

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-3813-1

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2016

  • Hardcover ISBN: 978-1-4939-3811-7Published: 18 July 2016

  • Softcover ISBN: 978-1-4939-8146-5Published: 31 May 2018

  • eBook ISBN: 978-1-4939-3813-1Published: 17 July 2016

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 3

  • Number of Pages: XIII, 314

  • Number of Illustrations: 16 b/w illustrations, 84 illustrations in colour

  • Topics: Biochemistry, general

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