Overview
- Editors:
-
-
That T. Ngo
-
Department of Developmental and Cell Biology, University of California, Irvine, USA
Access this book
Other ways to access
Table of contents (35 chapters)
-
Affinity Chromatography with Biomimetic Ligands
-
Front Matter
Pages 245-245
-
-
-
- T. William Hutchens, Tai-Tung Yip
Pages 277-312
-
- Milan J. Beneš, Alexandra Štambergova, William H. Scouten
Pages 313-322
-
- Tetsuya Tosa, Tadashi Sato, Taizo Watanabe, Satoshi Minobe
Pages 323-332
-
- Patrick Hubert, Edith Dellacherie
Pages 333-359
-
Novel Concepts and Applications
-
Front Matter
Pages 361-361
-
-
- Lars I. Andersson, Björn Ekberg, Klaus Mosbach
Pages 383-394
-
- Bo Mattiasson, Eva Linné, Rajni Kaul
Pages 395-401
-
-
- That T. Ngo, Neeta Khatter
Pages 415-430
-
Affinity-Related Techniques
-
Front Matter
Pages 431-431
-
- Shlomo Margel, Leon Marcus
Pages 433-450
-
- Shu-Ching Ma, Victor C. Yang
Pages 451-468
-
- Bo Mattiasson, Rajni Kaul
Pages 469-477
-
- Mathias Uhlén, Ørjan Olsvik, Erik Hornes
Pages 479-485
-
- Rajni Kaul, Bo Mattiasson
Pages 487-498
-
- Gerhard Kopperschläger, Gerd Birkenmeier
Pages 499-509
About this book
Rapid advances in molecular biology have accelerated the production of a great number of protein-based therapeutic agents. The major cost in producing these proteins appears to be associated with their purification from the complex mixture of the crude extract. A major challenge to the protein biochemist and the biochemical engineer is the development of rapid, efficient, and cost-effective purification systems. This volume presents state-of-the-art reviews of current methods used in the purifica tion of biological macromolecules that are based on molecular interactions. Thus, the major emphasis is placed on affinity-related techniques. Part I provides a general introduction to affinity chromatography and includes a chapter describing an interesting new technique called "slalom chromatography" for DNA fractionations. Affinity chromatography using molecules of biological origin as the affinity ligand is covered in Part II. Part III describes the use of a special class of biomolecules, antibodies, as affinity ligands. Affinity chromatography with biomimetic ligands is discussed in Part IV. Newer concepts and their applications in bioseparation are presented in Part V. Part VI covers affinity-related techniques such as affinity-based extracorporeal shunts, affinity electrophoresis, affinity precipitation, and affinity extraction. I would like to express my sincere thanks to all the authors, who are recognized experts in their respective fields, for their cooperation and contributions. I thank the editorial staff of Plenum Press for their professionalism, and Mary Phillips Born, Senior Editor, for her encouragement. The support of my family (Ping and Peilin) made it possible to complete editing this book. That T.
Editors and Affiliations
-
Department of Developmental and Cell Biology, University of California, Irvine, USA
That T. Ngo
