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Plant Proteostasis

Methods and Protocols

  • Book
  • © 2023

Overview

Editors:
  1. L. Maria Lois

    1. Center for Research in Agricultural Genomics (CSIC-IRTA-UAB-UB), Barcelona, Spain

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  2. Marco Trujillo

    1. Faculty of Biology, University of Freiburg, Freiburg, Germany

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    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Provides step-by-step detail essential for reproducible results

Part of the book series: Methods in Molecular Biology (MIMB, volume 2581)

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Table of contents (28 protocols)

  1. Protein Turnover and Stability

    1. Using Intrinsic Fluorescence to Measure Protein Stability Upon Thermal and Chemical Denaturation

      • Nathalia Varejão, David Reverter
      Pages 229-241

  2. Protein Isolation and Mass Spectrometric Analysis

    1. Front Matter

      Pages 243-243
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    2. Purification and Detection of Ubiquitinated Plant Proteins Using Tandem Ubiquitin Binding Entities

      • DongHyuk Lee, Gitta Coaker
      Pages 245-254

    3. Titanium Oxide-Based Phosphopeptide Enrichment from Arabidopsis Seedlings

      • Sharon C. Mithoe, Frank L. H. Menke
      Pages 255-265

    4. Chloroplast Envelope Membrane Subfractionation from Arabidopsis and Pea

      • Annabel Dischinger, Serena Schwenkert
      Pages 267-284

    5. Chromatin Enrichment for Proteomics in Plants (ChEP-P)

      • Isabel Cristina Vélez-Bermúdez, Wolfgang Schmidt
      Pages 285-293

    6. Proximity-Dependent In Vivo Biotin Labeling for Interactome Mapping in Marchantia polymorpha

      • Katharina Melkonian, Sara Christina Stolze, Anne Harzen, Hirofumi Nakagami
      Pages 295-308

    7. Tandem Mass Tag-Based Phosphoproteomics in Plants

      • Isabel Cristina Vélez-Bermúdez, Dharmesh Jain, Arya Ravindran, Chin-Wen Chen, Chuan-Chih Hsu, Wolfgang Schmidt
      Pages 309-319

  3. Study of Proteasome and Proteases

    1. Front Matter

      Pages 321-321
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    2. Analysis of Peptide Hormone Maturation and Processing Specificity Using Isotope-Labeled Peptides

      • Stefanie Brück, Jens Pfannstiel, Gwyneth Ingram, Annick Stintzi, Andreas Schaller
      Pages 323-335

    3. Improved Identification of Protease Cleavage Sites by In-gel Reductive Dimethylation

      • Stefanie Royek, Stefanie Brück, Jens Pfannstiel, Annick Stintzi, Andreas Schaller
      Pages 337-349

    4. A Pipeline to Monitor Proteasome Homeostasis in Plants

      • Gautier Langin, Suayib Ãœstün
      Pages 351-363

  4. Bioinformatic Analysis

    1. Front Matter

      Pages 365-365
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    2. Bioinformatic Tools for Exploring the SUMO Gene Network: An Update

      • Pedro Humberto Castro, Miguel Ângelo Santos, Alexandre Papadopoulos Magalhães, Rui Manuel Tavares, Herlander Azevedo
      Pages 367-383

    3. Coexpression Network Construction and Visualization from Transcriptomes Underlying ER Stress Responses

      • Dae Kwan Ko, Federica Brandizzi
      Pages 385-401

    4. Approaches for the Identification of Intrinsically Disordered Protein Domains

      • Huqiang Wang, Zhixiang Yang, Dong Yang
      Pages 403-412

  5. Back Matter

    Pages 413-415
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Keywords

About this book

 This second edition compiles state-of-art protocols to study the molecular mechanisms that determine proteome composition and contribute to maintaining protein homeostasis. Chapters describe methods that range from the in vitro analysis of enzyme cascades to real-time in vivo studies. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and cutting-edge, Plant Proteostasis: Methods and Protocols, Second Edition aims to be a useful practical guide to researches to help further their study in this field. 


Editors and Affiliations

  • Center for Research in Agricultural Genomics (CSIC-IRTA-UAB-UB), Barcelona, Spain

    L. Maria Lois

  • Faculty of Biology, University of Freiburg, Freiburg, Germany

    Marco Trujillo

Bibliographic Information

  • Book Title: Plant Proteostasis

  • Book Subtitle: Methods and Protocols

  • Editors: L. Maria Lois, Marco Trujillo

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-2784-6

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2023

  • Hardcover ISBN: 978-1-0716-2783-9Published: 23 November 2022

  • Softcover ISBN: 978-1-0716-2786-0Published: 23 November 2023

  • eBook ISBN: 978-1-0716-2784-6Published: 22 November 2022

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XVI, 415

  • Number of Illustrations: 23 b/w illustrations, 57 illustrations in colour

  • Topics: Plant Sciences

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