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G Protein-Coupled Receptor Screening Assays

Methods and Protocols

  • Book
  • © 2021

Overview

Editors:
  1. Sofia Aires M. Martins

    1. INESC Microsistemas e Nanotecnologias, Lisbon, Portugal

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    You can also search for this editor in PubMed   Google Scholar

  2. Duarte Miguel F. Prazeres

    1. IBB—Institute for Bioengineering and Biosciences, Lisbon, Portugal

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2268)

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Table of contents (21 protocols)

  1. Homology Modeling Using GPCRM Web Service

    • PrzemysÅ‚aw Miszta, Szymon NiewieczerzaÅ‚, PaweÅ‚ Pasznik, SÅ‚awomir Filipek
    Pages 305-321

  2. Correction to: Nanoluciferase-Based Complementation Assay to Detect GPCR-G Protein Interaction

    • Céline Laschet, Julien Hanson
    Pages C1-C2

  3. Back Matter

    Pages 323-327
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Keywords

About this book

This fully updated edition targets not only those assays directly involved in the discovery of GPCR-active compounds but also those involved in cell-based experiments designed to study physiological responses. Whether coming from academia or industry, or being an experienced researcher or a newcomer to the field, the reader will find accessible methods and protocols that cover the latest developments on receptor purification, molecular biology, recombinant engineering, and analytical techniques that enable the real time monitoring of the complex GPCR signaling cascade and identification of potential drug targets. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and up-to-date, G Protein-Coupled Receptor Screening Assays: Methods and Protocols, Second Edition aims to provide the tools necessary to contribute to the advancement of GPCR research and discovery and ultimately lead to the availability of innovative and more efficient drugs.

Editors and Affiliations

  • INESC Microsistemas e Nanotecnologias, Lisbon, Portugal

    Sofia Aires M. Martins

  • IBB—Institute for Bioengineering and Biosciences, Lisbon, Portugal

    Duarte Miguel F. Prazeres

Bibliographic Information

  • Book Title: G Protein-Coupled Receptor Screening Assays

  • Book Subtitle: Methods and Protocols

  • Editors: Sofia Aires M. Martins, Duarte Miguel F. Prazeres

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-1221-7

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2021

  • Hardcover ISBN: 978-1-0716-1220-0Published: 05 June 2021

  • Softcover ISBN: 978-1-0716-1223-1Published: 06 June 2022

  • eBook ISBN: 978-1-0716-1221-7Published: 04 June 2021

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XVI, 327

  • Number of Illustrations: 9 b/w illustrations, 69 illustrations in colour

  • Topics: Membrane Biology, Pharmacology/Toxicology, Chemistry/Food Science, general

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