Overview
- Editors:
-
-
Willem F. Wolkers
-
Unit for Reproductive Medicine—Clinic for Horses, Biostabilization Laboratory—Lower Saxony Centre for Biomedical Engineering, Implant Research and Development, University of Veterinary Medicine Hannover, Hannover, Germany, University of Veterinary Medicine Hannover, Hannover, Germany
-
Harriëtte Oldenhof
-
Unit for Reproductive Medicine—Clinic for Horses, University of Veterinary Medicine Hannover, Hannover, Germany
- Includes cutting-edge techniques
- Provides step-by-step detail essential for reproducible results
- Contains key implementation advice from the experts
Access this book
Other ways to access
Table of contents (41 protocols)
-
Cryopreservation and Freeze-Drying Protocols
-
-
- Estefania Paredes, Pablo Heres, Catarina Anjos, Elsa Cabrita
Pages 413-425
-
- Mengying Wang, Evgenia Isachenko, Gohar Rahimi, Peter Mallmann, Vladimir Isachenko
Pages 427-436
-
- Victoria Keros, Barry J. Fuller
Pages 437-454
-
- Tamas Somfai, Kazuhiro Kikuchi
Pages 455-468
-
-
- Jana Liebenthron, Markus Montag
Pages 485-499
-
-
-
-
- Denys Pogozhykh, Rainer Blasczyk, Constança Figueiredo
Pages 539-554
-
-
- Juliette Seremak, Ali Eroglu
Pages 569-579
-
- Leah A. Marquez-Curtis, Nasim Eskandari, Locksley E. McGann, Janet A. W. Elliott
Pages 581-591
-
- Kelvin G. M. Brockbank, Zhenzhen Chen, Elizabeth D. Greene, Lia H. Campbell
Pages 593-605
-
- Estefania Paredes, Angela Ward, Ian Probert, Léna Gouhier, Christine N. Campbell
Pages 607-621
-
- Anna Nebot, Victoria J. Philpott, Anna Pajdo, Daniel Ballesteros
Pages 623-637
-
- Heinz Martin Schumacher, Martina Westphal, Elke Heine-Dobbernack
Pages 639-645
-
- Angelika Senula, Manuela Nagel
Pages 647-661
-
- Daniel Ballesteros, Natalia Fanega-Sleziak, Rachael M. Davies
Pages 663-682
About this book
This fourth edition explores fully up-to-date standardly used cryopreservation, vitrification, and freeze-drying protocols for specimens that are used for research purposes, conservation of genetic reserves, and applications in agriculture and medicine. Beginning with a section on the fundamentals as well as the use of mathematical modeling to solve cryobiological problems, the book continues with sections on technological aspects of freezing and drying, analytical methods to study protectant loading of cells and tissues, cell behavior during freezing and drying, and thermodynamic properties of preservation solutions, as well as cryopreservation, vitrification, and freeze-drying protocols for a wide variety of samples and different applications. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Thorough and authoritative, Cryopreservation and Freeze-Drying Protocols, Fourth Edition serves as an ideal guide for researchers and technical assistants in academia and industry with a background in life sciences, medicine, or engineering who want to investigate freezing and drying of biological systems or set up methods to safely store biological specimens while maintaining their function upon reconstitution.
The chapter “Freezing Technology: Control of Freezing, Thawing, and Ice Nucleation” is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
Editors and Affiliations
-
Unit for Reproductive Medicine—Clinic for Horses, Biostabilization Laboratory—Lower Saxony Centre for Biomedical Engineering, Implant Research and Development, University of Veterinary Medicine Hannover, Hannover, Germany, University of Veterinary Medicine Hannover, Hannover, Germany
Willem F. Wolkers
-
Unit for Reproductive Medicine—Clinic for Horses, University of Veterinary Medicine Hannover, Hannover, Germany
Harriëtte Oldenhof