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Molecular Dermatology

Methods and Protocols

  • Book
  • © 2020

Overview

Editors:
  1. ​Natalia V. Botchkareva

    1. Monasterium Laboratory Skin and Hair Research Solutions GmbH, Münster, Germany

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    You can also search for this editor in PubMed   Google Scholar

  2. Gillian E. Westgate

    1. Centre for Skin Sciences, University of Bradford, Bradford, UK

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2154)

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Table of contents (22 protocols)

  1. Front Matter

    Pages i-x
    Download chapter PDF

  2. Isolation of Epidermal Keratinocytes from Human Skin: The Scratch-Wound Assay for Assessment of Epidermal Keratinocyte Migration

    • Irene Castellano-Pellicena, M. Julie Thornton
    Pages 1-12

  3. Isolation of Different Dermal Fibroblast Populations from the Skin and the Hair Follicle

    • Rachael Williams, M. Julie Thornton
    Pages 13-22

  4. Isolation of Epidermal and Hair Follicle Melanocytes

    • Richard Baker, M. Julie Thornton
    Pages 23-32

  5. Isolation and Culture of Human Skin Mast Cells

    • Hanna Siiskonen, Jörg Scheffel
    Pages 33-43

  6. Organotypic 3D Skin Models: Human Epidermal Equivalent Cultures from Primary Keratinocytes and Immortalized Keratinocyte Cell Lines

    • Gijs Rikken, Hanna Niehues, Ellen H. van den Bogaard
    Pages 45-61

  7. Purification of Extracellular Microvesicles Secreted by Dermal Fibroblasts

    • Isabelle Petit, Ayelet Levy, Daniel Aberdam
    Pages 63-72

  8. A Method to Investigate the Epidermal Permeability Barrier In Vitro

    • Yoshikazu Uchida, Anna Celli
    Pages 73-90

  9. Isolating Dermal Papilla Cells from Human Hair Follicles Using Microdissection and Enzyme Digestion

    • Summik Limbu, Claire A. Higgins
    Pages 91-103

  10. Methods to Study Human Hair Follicle Growth Ex Vivo: Human Microdissected Hair Follicle and Human Full Thickness Skin Organ Culture

    • Janin Edelkamp, Jennifer Gherardini, Marta Bertolini
    Pages 105-119

  11. Nonsurgical Induction of Alopecia Areata in C3H/HeJ Mice via Adoptive Transfer of Cultured Lymphoid Cells

    • Eddy H. C. Wang, Kevin J. McElwee
    Pages 121-131

  12. Collapse and Restoration of Hair Follicle Immune Privilege Ex Vivo: A Model for Alopecia Areata

    • Markus Fehrholz, Marta Bertolini
    Pages 133-141

  13. Experimentally Induced Epithelial–Mesenchymal Transition of Human Hair Follicle Stem Cells as a Model of Scarring Alopecia Ex Vivo

    • Ilaria Piccini, Marta Bertolini
    Pages 143-152

  14. Generation of Hair Follicle Germs In Vitro Using Human Postnatal Skin Cells

    • Ekaterina P. Kalabusheva, Ekaterina A. Vorotelyak
    Pages 153-163

  15. Method for Human Eccrine Sweat Gland Isolation from the Scalp by Means of the Micropunch Technique and Vital Dyes

    • Francisco Jiménez
    Pages 165-173

  16. Identification of Long Noncoding RNA by In Situ Hybridization Approaches

    • Mara Mancini, Anna Maria Lena, Eleonora Candi
    Pages 175-185

  17. Detection of MicroRNAs by In Situ Hybridization in Skin

    • Maximilian E. Pickup, Mohammed I. Ahmed
    Pages 187-196

  18. Chromatin Immunoprecipitation of Low Number of FACS-Purified Epidermal Cells

    • Carmit Bar, V. Julian Valdes, Elena Ezhkova
    Pages 197-215

  19. 3D-FISH Analysis of the Spatial Genome Organization in Skin Cells in Situ

    • Andrei N. Mardaryev, Michael Y. Fessing
    Pages 217-230

  20. Method to Study Skin Cancer : Two-Stage Chemically Induced Carcinogenesis in Mouse Skin

    • Andrei N. Mardaryev
    Pages 231-238
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Keywords

About this book

This volume delivers the most important laboratory techniques that underlie dermatological investigation, including in situ, in vitro, ex vivo, and in vivo models of skin, its appendages (hair follicles, sebaceous and sweat glands), and immune cells. Also included are unique techniques that unravel epigenetic mechanisms in skin and hair follicle cells, such as gene organization in the nucleus and gene expression regulation by the non-coding transcriptome, which are important recent developments in investigative dermatology. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Molecular Dermatology: Methods and Protocols serves as an essential resource for researchers working in the areas of fundamental as well as applied dermatological science.

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Editors and Affiliations

  • Monasterium Laboratory Skin and Hair Research Solutions GmbH, Münster, Germany

    ​Natalia V. Botchkareva

  • Centre for Skin Sciences, University of Bradford, Bradford, UK

    Gillian E. Westgate

Bibliographic Information

  • Book Title: Molecular Dermatology

  • Book Subtitle: Methods and Protocols

  • Editors: ​Natalia V. Botchkareva, Gillian E. Westgate

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-0648-3

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020

  • Hardcover ISBN: 978-1-0716-0647-6Published: 21 April 2020

  • Softcover ISBN: 978-1-0716-0650-6Published: 21 April 2021

  • eBook ISBN: 978-1-0716-0648-3Published: 20 April 2020

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 278

  • Number of Illustrations: 6 b/w illustrations, 69 illustrations in colour

  • Topics: Biochemistry, general, Dermatology, Cell Biology

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