Methods in Biotechnology

Environmental Microbiology

Methods and Protocols

Editors: Spencer, John F. T., Alicia L. Ragout de Spencer (Eds.)

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About this book

Understanding the relationship between a microorganism and its environment is essential to the successful manipulation of industrial, biochemical, and medical processes. In Environmental Microbiology: Methods and Protocols, highly practiced experimentalists who often have perfected the methods they write about describe readily reproducible techniques for determining most of the important factors governing microorganisms and their habitats. Presented in step-by-step detail, these cutting-edge methods range from those for the study of marine organisms, to those for investigating microorganisms occurring in groundwater, to the biodiversity found in remote environments. The protocols for studying fermented milks are significant for investigators concerned with milk as an item of food for infants, small children, and even adults. Additional methods for the recovery and determination of nucleic acids and other compounds affecting, and affected by, microorganisms, are provided for certain enzymes produced by plant pathogens and for obtaining microbial species tolerant of such inhibitors as heavy metals. Review articles discuss the endophytic bacterium Bacillus mojavensis, the engineering of bacteria to enhance their ability to carry out bioremediation of aromatic compounds, and the use of chemical shift reagents and Na-NMR to study sodium gradients in microorganisms. The protocols follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
State-of-the-art and highly practical, Environmental Microbiology: Methods and Protocols offers microbiological researchers a powerful set of techniques for investigating and understanding microorganisms in their native environments.

Table of contents (38 chapters)

  • Isolation and Molecular Characterization of Seawater Bacteria

    Benito, Juliana M. (et al.)

    Pages 3-9

  • Reverse Sample Genome Probing to Monitor Microbial Communities

    Greene, E. Anne (et al.)

    Pages 11-22

  • T-RFLP Analysis

    Liesack, Werner (et al.)

    Pages 23-37

  • Assessing Bacterial DNA Content in Aquatic Systems

    Pernèy, Garrett W. (et al.)

    Pages 39-50

  • Multiplexed Identification and Quantification of Analyte DNAs in Environmental Samples Using Microspheres and Flow Cytometry

    Lowe, Mary (et al.)

    Pages 51-74

Buy this book

eBook $159.00
price for USA (gross)
  • ISBN 978-1-59259-765-9
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $209.00
price for USA
  • ISBN 978-1-58829-116-5
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $209.00
price for USA
  • ISBN 978-1-61737-370-1
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Environmental Microbiology
Book Subtitle
Methods and Protocols
Editors
  • John F. T. Spencer
  • Alicia L. Ragout de Spencer
Series Title
Methods in Biotechnology
Series Volume
16
Copyright
2004
Publisher
Humana Press
Copyright Holder
Humana Press
eBook ISBN
978-1-59259-765-9
DOI
10.1385/1592597653
Hardcover ISBN
978-1-58829-116-5
Softcover ISBN
978-1-61737-370-1
Series ISSN
1940-6061
Edition Number
1
Number of Pages
XIV, 426
Topics