Overview
- Editors:
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Yao-Shan Fan
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London Health Sciences Center, London
The University of Western Ontario, Ontario, Canada
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Table of contents (27 protocols)
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Special Applications in Chromosomal Disorders
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- Farideh Z. Bischoff, Dianne X. Dang, Dorothy E. Lewis, Joe Leigh Simpson
Pages 243-258
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- Yury Verlinsky, Jeanine Cieslak, Anver Kuliev
Pages 259-273
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- Stuart Schwartz, Michael D. Graf
Pages 275-290
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- Héléne Bruyére, Evica Rajcan-Separovic, Dagmar K. Kalousek
Pages 299-307
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Special Applications in Oncology
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Front Matter
Pages 309-309
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- Bae-Li Hsi, Sheng Xiao, Jonathan A. Fletcher
Pages 343-351
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- Rosemary E. Mueller, Frances P. O’Malley
Pages 353-367
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- Marjan M. Weiss, Mari A. J. A. Hermsen, Gerrit A. Meijer, Paul J. van Diest
Pages 369-378
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- Yanming Zhang, Brigitte Schlegelberger
Pages 379-390
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- Xiao-Ning Chen, Julie R. Korenberg
Pages 391-403
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Back Matter
Pages 405-411
About this book
The new techniques of molecular cytogenetics, mainly fluorescence in situ hybridization (FISH) of DNA probes to metaphase chromosomes or interphase nuclei, have been developed in the past two decades. Many FISH techniques have been implemented for diagnostic services, whereas some others are mainly used for investigational purposes. Several hundreds of FISH probes and hybridization kits are now commercially available, and the list is growing rapidly. FISH has been widely used as a powerful diagnostic tool in many areas of medicine including pediatrics, medical genetics, maternal–fetal medicine, reproductive medicine, pathology, hematology, and oncology. Frequently, a physician may be puzzled by the variety of FISH techniques and wonder what test to order. It is not uncommon that a sample is referred to a laboratory for FISH without indicating a specific test. On the other hand, a cytogeneticist or a technologist in a laboratory needs, from case to case, to determine which procedure to perform and which probe to use for an informative result. To obtain the best results, one must use the right DNA probes and have reliable protocols and measures of quality assurance in place. Also, one must have sufficient knowledge in both traditional and molecular cytogenetics, as well as the particular areas of medicine for which the test is used in order to appropriately interpret the FISH results, and to correlate them with clinical diagnosis, treatment, and prognosis.
Reviews
"Dr. Fan has done a marvelous job of putting together a collection of laboratory protocols and review articles of different FISH technologies...Molecular Cytogenetics: Protocols and Applications is packed with information on cutting-edge FISH technologies and their applications, limitations, and pitfalls, along with detailed laboratory protocols. I would certainly recommend that this book be included in the libraries of cytogeneticists and cytogenetic laboratories" - Am. J. Hum. Gent.
"Dr. Fan has done a marvelous job of putting together a collection of laboratory protocols and review articles of different FISH technologies. The authors of the different chapters not only gave detailed, easy-to-follow protocols, but also provided their first-hand experiences of troubleshooting. Molecular Cytogenetics: Protocols and Applications is packed with information on cutting-edge FISH technologies and their applications, limitations, and pitfalls, along with detailed laboratory protocols. I would certainly recommend that this book be included in the libraries of cytogeneticists and cytogenetic laboratories." -The American Society of Human Genetics
Editors and Affiliations
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London Health Sciences Center, London
Yao-Shan Fan
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The University of Western Ontario, Ontario, Canada
Yao-Shan Fan