Overview
- Editors:
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José M. Martinez-Zapater
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Centro de Investigación y Tecnologia, Instituto Nacional de Investigación y Tecnologia Agraria y Alimentaria, Madrid, Spain
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Julio Salinas
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Centro de Investigación y Tecnologia, Instituto Nacional de Investigación y Tecnologia Agraria y Alimentaria, Madrid, Spain
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Table of contents (40 protocols)
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Mutagenesis and Genetic Analysis
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- Rodney G. Winkler, Kenneth A. Feldmann
Pages 129-136
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Gene Mapping in Arabidopsis
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Front Matter
Pages 135-135
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- Carlos Alonso-Blanco, Maarten Koornneef, Piet Stam
Pages 137-146
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- Jane Glazebrook, Eliana Drenkarci, Daphne Preuss, Frederick M. Ausubel
Pages 173-182
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- Francis D. Agyare, Gus Lagos, Deval Lashkari, Ronald W. Davis, Bertrand Lemieux
Pages 199-208
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Transient and Stable Transformation
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Front Matter
Pages 207-207
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- Steffen Abel, Athanasios Theologis
Pages 209-217
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- Motoaki Seki, Asako Lida, Hiromichi Morikawa
Pages 219-225
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- Annette C. Vergunst, Ellen C. de Waal, Paul J. J. Hooykaas
Pages 227-244
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- Eric van der Graaff, Paul J. J. Hooykaas
Pages 245-258
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- Nicole Bechtold, Georges Pelletier
Pages 259-266
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- Jaideep Mathur, Csaba Koncz
Pages 267-276
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VI Gene Cloning Strategies
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Front Matter
Pages 275-275
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- Jeffrey Leung, Jéréme Giraudat
Pages 277-303
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- Ann Van Gysel, Gerda Cnops, Peter Breyne, Marc Van Montagu, Maria Teresa Cervera
Pages 305-314
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- Deborah Long, George Coupland
Pages 315-328
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- Andy Pereira, Mark G. M. Aarts
Pages 329-338
About this book
The use of Arabidopsis in genetics and biology laboratories can be traced back to the beginning of the century, when the small size of this plant species, its short generation time, and the thousands of seeds produced by each plant attracted the interest of geneticists. In the middle of the century, this plant had already gained a place in several genetics laboratories both in Europe and the United States, being widely used in mutagenesis experiments. However, exponential growth in the use of Arabidopsis as a model system did not get started until the 1980s, when the small size of its genome was realized, thus triggering the interest of geneticists and molecular biologists, not only from the plant field, but from other research fields needing a novel model system. In recent years, high expectations for Plant Biotechnology in the next century, together with the need for basic information in every area of plant biology, have served to mobilize resources and orient much new research towards the plant field, where the special biological features of Arabidopsis have further focused attention on this useful species. The high level of interest in Arabidopsis has worked as a catalyst and, today, the system has been dev- oped to the point where any gene identified on the basis ofa phenotype can be cloned with a reasonable amount of time and effort.
Reviews
"...especially valuable for detailed step-by-step procedures including many important notes...an indispensable guide, which should be at reach on the shelf in each laboratory where the plant molecular-genetic methods in Arabidopsis, and not only Arabidopsis, are used."- Biologia Plantarum, Reviewer: J. Relichová
". . .a comprehensive collection of significant new methods as well as updated standard protocols. . .written by experienced authors and internationally recognized experts."-Journal of Plant Physiology