Overview
- Editors:
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Jac A. Nickoloff
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Department of Cancer Biology, Harvard University School of Public Health, Boston
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Table of contents (35 protocols)
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- Thea W. Aukrust, May B. Brurberg, Ingolf F. Nes
Pages 201-208
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- Brett D. Shepard, Michael S. Gilmore
Pages 217-226
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- Lisa Stowers, James Gautsch, Richard Dana, Merl F. Hoekstra
Pages 261-267
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- Martin Grey, Martin Brendel
Pages 269-272
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- Mark T. Hood, C. S. Stachow
Pages 273-278
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- Dietmar Becher, Stephen G. Oliver
Pages 291-302
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- Timothy G. Burland, Juliet Bailey
Pages 303-320
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- David Knecht, Ka Ming Pang
Pages 321-330
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- Jacek Gaertig, Martin A. Gorovsky
Pages 331-348
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- John M. Kelly, Martin C. Taylor, Gloria Rudenko, Pat A. Blundell
Pages 349-359
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- A. L. Wang, Tiina Sepp, C. C. Wang
Pages 361-367
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Back Matter
Pages 369-372
About this book
Electroporation is one of the most widespread techniques used in modem molecular genetics. It is most commonly used to introduce DNA into cells for investigations of gene structure and function, and in this regard, electroporation is both highly versatile, being effective with nearly all species and cell types, and highly efficient. For many cell types, electroporation is either the most efficient or the only means known to effect gene transfer. However, exposure of cells to brief, hi- intensity electric fields has found broad application in other aspects of biological research, and is now routinely used to introduce other types of biological and analytic molecules into cells, to induce cell-cell fusion, and to transfer DNA directly between different species. The first seven chapters of Electroporation Protocols for Micro organisms describe the underlying theory of electroporation, the com mercially available instrumentation, and a number of specialized electroporation applications, such as cDNA library construction and interspecies DNA electrotransfer. Each of the remaining chapters pre sents a well developed method for electrotransformation of a particular bacterial, fungal, or protist species. These chapters also serve to intro duce those new to the field the important research questions that are currently being addressed with particular organisms, highlighting both the major advantages and limitations of each species as a model organ ism, and explaining the roles that electroporation has played in the development of the molecular genetic systems currently in use.
Reviews
...the most comprehensive treatment available on the topic of electroporation of microorganisms...will be a useful resource for scientists working on diverse aspects of microbial genetics.-Four Stars, Doody's Health Sciences Book Review Journal
Editors and Affiliations
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Department of Cancer Biology, Harvard University School of Public Health, Boston
Jac A. Nickoloff