Overview
- Offers a comprehensive, up-to-date collection of all the main strategies of experimental genome-scale gene essentiality analysis
- Contains a detailed description of the procedures as well as a discussion of potential difficulties and failures
- Includes chapters on statistical analysis of the generated datasets and their integration into existing genomic databases
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 416)
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Table of contents (31 protocols)
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Introduction
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Experimental Protocols
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Populational Genome-Wide Essentiality Screens
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Systematic Collections of Knockout Mutants
Keywords
About this book
Microbial Gene Essentiality: Protocols and Bioinformatics contains a comprehensive collection of experimental and computational strategies and techniques for microbial genome-scale essentiality studies, developed and presented by the leading groups in the field. In addition to wet-lab protocols, the book describes (i) statistical methods essential for planning successful large-scale essentiality screens, as well for data evaluation and analysis; (ii) in-silico prediction of gene essentiality using genome-scale reconstructed metabolic models; and (iii) data integration and comparative analysis in the context of genomic databases. This volume provides researchers with a first-stop guide for choosing the most appropriate strategy for planned essentiality studies. Experimental and computational aspects are equally important in genome-scale gene essentiality analysis, as in all other genomic technologies, and Microbial Gene Essentiality: Protocols and Bioinformatics reflects both of these aspects. All protocols follow the successful Methods in Molecular Biologyâ„¢ series format, each offering an introduction outlining the principles behind the techniques, step-by-step instructions, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding pitfalls, and are intended for both novice and expert scientists.
Editors and Affiliations
Bibliographic Information
Book Title: Microbial Gene Essentiality: Protocols and Bioinformatics
Editors: Andrei L. Osterman, Svetlana Y. Gerdes
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-59745-321-9
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Humana Press 2008
Hardcover ISBN: 978-1-58829-378-7Published: 04 February 2008
Softcover ISBN: 978-1-61737-529-3Published: 19 November 2010
eBook ISBN: 978-1-59745-321-9Published: 08 April 2008
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XIV, 486
Topics: Biochemistry, general, Cell Biology, Human Genetics