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Reverse Phase Protein Arrays

From Technical and Analytical Fundamentals to Applications

  • Book
  • © 2019

Overview

Editors:
  1. Tesshi Yamada

    1. Division of Cellular Signaling, National Cancer Center Research Institute, Tokyo, Japan

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  2. Satoshi S. Nishizuka

    1. Division of Biomedical, Research and Development, Institute of Biomedical Science, Iwate Medical University, Yahaba, Iwate, Japan

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  3. Gordon B. Mills

    1. Department of Systems Biology, The University of Texas MD Anderson Cancer Center, Houston, USA

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  4. Lance A. Liotta

    1. Center for Applied Proteomics and Molecular Medicine, George Mason University, Manassas, USA

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  • Guidance on handling of samples for stable data generation
  • Explanation of the role of RPPA data in integrative analyses
  • Expert opinions and updates on recent results from RPPA

Part of the book series: Advances in Experimental Medicine and Biology (AEMB, volume 1188)

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Table of contents (14 chapters)

  1. Front Matter

    Pages i-viii
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  2. RPPA: Origins, Transition to a Validated Clinical Research Tool, and Next Generations of the Technology

    • Emanuel Petricoin III, Julie Wulfkuhle, Marissa Howard, Marielena Pierobon, Virginia Espina, Alessandra Luchini et al.
    Pages 1-19

  3. Lysate Preparation for Reverse Phase Protein Arrays

    • Karl-Friedrich Becker
    Pages 21-30

  4. High Precision RPPA: Concept, Features, and Application Performance of the Integrated Zeptosens Platform

    • Gerhard M. Kresbach, Michael Pawlak
    Pages 31-59

  5. Solid Pin Protein Array Printing Platforms

    • Virginia Espina, Claudius Mueller
    Pages 61-75

  6. Analytical Platforms 1: Use of Cultured Cells and Fluorescent Read-Out Coupled to NormaCurve Normalization in RPPA

    • Bérengère Ouine, Sabine Rajkumar, Philippe Hupé, Leanne de Koning
    Pages 77-93

  7. Combining the “Sibling Technologies” of Laser Capture Microdissection and Reverse Phase Protein Microarrays

    • Claudius Mueller, Justin B. Davis, Lance A. Liotta
    Pages 95-111

  8. Analytical Platforms 3: Processing Samples via the RPPA Pipeline to Generate Large-Scale Data for Clinical Studies

    • Doris R. Siwak, Jun Li, Rehan Akbani, Han Liang, Yiling Lu
    Pages 113-147

  9. Antibody Screening

    • Fieke W. Hoff, Yiling Lu, Steven M. Kornblau
    Pages 149-163

  10. Generation of Raw RPPA Data and Their Conversion to Analysis-Ready Data

    • Rehan Akbani, Shiyun Ling, Yiling Lu
    Pages 165-180

  11. Reproducibility and Crossplatform Validation of Reverse-Phase Protein Array Data

    • Adam Byron
    Pages 181-201

  12. Drug Screening Platforms and RPPA

    • John C. Dawson, Scott J. Warchal, Neil O. Carragher
    Pages 203-226

  13. RPPAs for Cell Subpopulation Analysis

    • Kohei Kume, Satoshi S. Nishizuka
    Pages 227-237

  14. Utility of Reverse-Phase Protein Array for Refining Precision Oncology

    • Mari Masuda, Tesshi Yamada
    Pages 239-249

  15. Using Reverse Phase Protein Array (RPPA) to Identify and Target Adaptive Resistance

    • Marilyne Labrie, Yong Fang, Nicholas D. Kendsersky, Jun Li, Han Liang, Shannon N. Westin et al.
    Pages 251-266
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Keywords

About this book

This book provides practical guidance on all aspects of reverse phase protein array (RPPA) technology, which permits the quantification of protein levels in cell or tissue lysates. In addition, the latest results are presented from laboratories across the world where experts are successfully running the challenging RPPA data platform. After an introductory chapter by the inventor of the RPPA technique, the demanding task of lysate preparation is explained. Subsequent chapters review printing platforms and analytical platforms and discuss antibody screening in detail. The role of RPPA data in integrative analyses is thoroughly examined, and the various applications of RPPA, for example to identify molecular targeting drugs or evaluate cancer drug efficacy, are discussed with reference to the latest knowledge. It is hoped that the book will foster the implementation of RPPA by documenting the practical details of the technology and by revealing its great potential. It will be of high value for researchers who plan to build an RPPA platform in their institute and for researchers, clinicians, and companies who use RPPA through collaboration with an existing RPPA facility.


Editors and Affiliations

  • Division of Cellular Signaling, National Cancer Center Research Institute, Tokyo, Japan

    Tesshi Yamada

  • Division of Biomedical, Research and Development, Institute of Biomedical Science, Iwate Medical University, Yahaba, Iwate, Japan

    Satoshi S. Nishizuka

  • Department of Systems Biology, The University of Texas MD Anderson Cancer Center, Houston, USA

    Gordon B. Mills

  • Center for Applied Proteomics and Molecular Medicine, George Mason University, Manassas, USA

    Lance A. Liotta

About the editors

Tesshi Yamada, M.D., Ph.D. 

Professor of International University of Health and Welfare, Narita, Japan


Satoshi Nishizuka, M.D., Ph.D. 

Professor of Iwate Medical University, Japan


Gordon Mills, M.D., Ph.D. 

Professor of University of Texas, MD Anderson Cancer Center


Lance Liotta, M.D., Ph.D. 

Co-Director of the Center for Applied Proteomics and Molecular Medicine, George Mason University

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