Springer Theses

Acoustic Levitation-Based Trace-Level Biosensing

Design of Detection Systems and Applications to Real Samples

Authors: Miyagawa, Akihisa

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  • Nominated as an outstanding Ph.D. thesis by the Tokyo Institute of Technology
  • Proposes a novel trace detection concept based on a coupled acoustic-gravitational field
  • Analyzes the kinetics and thermodynamics of complex reactions that cannot be evaluated by other methods
  • Enables readers to detect various bioanalytical targets by simple modifications of the method
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eBook $149.00
price for USA in USD
  • ISBN 978-981-16-1425-5
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  • Immediate eBook download after purchase
Hardcover $199.99
price for USA in USD
  • ISBN 978-981-16-1424-8
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  • Covid-19 shipping restrictions
  • Usually ready to be dispatched within 3 to 5 business days, if in stock
About this book

This book shows the availability and potential of the coupled acoustic-gravitational (CAG) field for trace-level biosensing. The proposed detection scheme also allows the evaluation of the kinetics and thermodynamics of the reaction occurring on a single microparticle (MP). This method has wide applicability in important fields, involving not only chemistry but also life, environmental, and medical sciences. 
The author proposes novel trace-level biosensing based on measurements of the levitation coordinate shift of an MP in the CAG field. The levitation coordinate of the MP in the CAG field is determined by its density and compressibility. The levitation coordinate shift is induced by the binding of gold nanoparticles (AuNPs) to the MP through interparticle reactions. Therefore, the quantity of molecules involved in the reaction can be determined from the levitation coordinate shift. The author demonstrates the zmol level detection for biotin, DNA/RNA, and organic molecules. In addition, the kinetics and thermodynamics are evaluated for various reactions occurring between the MP and AuNP, such as the avidin-biotin reaction, direct hybridization, sandwich hybridization, and aptamer-target complexation.
This book provides a new concept based on the CAG field, in which the extent of a reaction is converted into the levitation coordinate shift, that is, “length.” The proposed method has many advantages over other methods, e.g., high biocompatibility, high applicability, and short analysis time. In addition, because the apparatus used in this study is inexpensive and easy to miniaturize, this method is useful in important practical fields, such as forensic and environmental science and diagnosis. Thus, this book inspires many researchers to apply the present method to their own fields of interest.

About the authors

Akihisa Miyagawa is an Assistant Professor at Tsukuba University. He gained his Ph.D. under the supervision of Professor Tetsuo Okada at Tokyo Institute of Technology in 2020. His Ph.D. research subject was “Acoustic Levitation-Based Trace-Level Biosensing. Design of Detection Systems and Applications to Real Samples”. He is interested in Analytical Chemistry, Molecular Spectroscopy, Chemistry under the Hydrostatic Pressure, and Interfacial Chemistry.

Table of contents (7 chapters)

Table of contents (7 chapters)

Buy this book

eBook $149.00
price for USA in USD
  • ISBN 978-981-16-1425-5
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $199.99
price for USA in USD
  • ISBN 978-981-16-1424-8
  • Free shipping for individuals worldwide
  • Institutional customers should get in touch with their account manager
  • Covid-19 shipping restrictions
  • Usually ready to be dispatched within 3 to 5 business days, if in stock
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Bibliographic Information

Bibliographic Information
Book Title
Acoustic Levitation-Based Trace-Level Biosensing
Book Subtitle
Design of Detection Systems and Applications to Real Samples
Authors
Series Title
Springer Theses
Copyright
2021
Publisher
Springer Singapore
Copyright Holder
The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Nature Singapore Pte Ltd.
eBook ISBN
978-981-16-1425-5
DOI
10.1007/978-981-16-1425-5
Hardcover ISBN
978-981-16-1424-8
Series ISSN
2190-5053
Edition Number
1
Number of Pages
XII, 93
Number of Illustrations
18 b/w illustrations, 44 illustrations in colour
Topics