Overview
- Nominated as an outstanding PhD thesis by Kyoto University
- Is the first-ever report on three elegant designs of cooperative DNA-binding systems
- Highlights the most important aspects of programmable DNA-binding pyrrole–imidazole polyamides for quick learning
Part of the book series: Springer Theses (Springer Theses)
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Table of contents (4 chapters)
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Front Matter
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Back Matter
Keywords
About this book
This book presents three types of synthetically cooperative DNA recognizing assemblies, in order to advance the development of programmable DNA-binding pyrrole–imidazole polyamides (PIPs).
PIPs represent the best-characterized class of small molecule DNA binders that can be modified to bind with any predetermined DNA sequence and regulate gene expression patterns in a transgene-free and cost-effective manner. PIPs are characterized by their small molecular size, high binding affinity, programmability, sequence selectivity, and moderate cell permeability. In recent years, there have been numerous novel studies on the applications of these biological tools; this research is thoroughly reviewed in the first chapter.
There are several critical issues, however, that impede the further broad study of PIPs, which greatly concern the author. For instance, the short PIP version has an excessively hi^10 bp; this significantly decreases cell permeability. Moreover, the conventional binding strategy for PIP design cannot apply to flexible DNA binding—for example, the DNA-binding mode of a transcription factor pair.
In this book, the author describes the development of three kinds of cooperative DNA-binding systems that help resolve the current highly problematic issues concerning PIPs. These three systems offer a range of significant advantages, such as favorable sequence selectivity, long recognition sequence, higher binding affinity, and a flexible gap distance.
Released at a critical juncture in the application of PIPs, this book will greatly facilitate their use as therapeutic drugs in the treatment of cancer and hereditary diseases, and in regenerative medicine.
Authors and Affiliations
Bibliographic Information
Book Title: Artificial Assemblies with Cooperative DNA Recognition
Authors: Zutao YU
Series Title: Springer Theses
DOI: https://doi.org/10.1007/978-981-15-4423-1
Publisher: Springer Singapore
eBook Packages: Chemistry and Materials Science, Chemistry and Material Science (R0)
Copyright Information: Springer Nature Singapore Pte Ltd. 2020
Hardcover ISBN: 978-981-15-4422-4Published: 10 May 2020
Softcover ISBN: 978-981-15-4425-5Published: 11 May 2021
eBook ISBN: 978-981-15-4423-1Published: 09 May 2020
Series ISSN: 2190-5053
Series E-ISSN: 2190-5061
Edition Number: 1
Number of Pages: XV, 136
Number of Illustrations: 17 b/w illustrations, 119 illustrations in colour
Topics: Biotechnology, Bioorganic Chemistry, Nucleic Acid Chemistry, Medicinal Chemistry