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Rhizosphere Biology

Hairy Root Cultures Based Applications

Methods and Protocols

Editors: Srivastava, Vikas, Mehrotra, Shakti, Mishra, Sonal (Eds.)

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  • Discusses how Agrobacterium has revolutionized plant genetic engineering
  • Provides specific case studies on the utilization of hairy root in metabolite production
  • Describes plant-microbe interaction with respect to A. rhizogenes
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  • ISBN 978-981-15-4055-4
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About this book

The book discusses the various methods and protocols available in hairy root culture-based research. The utilization of Agrobacterium mediated genetic transformation and establishment of hairy root cultures has paved the way for large-scale secondary metabolite production in medicinal plants. Presenting recent research and offering insights from eminent research groups, the book covers a range of topics related to hairy root-based applications, including (i) establishment of hairy roots and native production of SM (ii) yield enhancement strategies for increased SM production, like elicitation (iii) hairy roots as a tool for value-added applications such as plant-microbe interaction, characterization of plant genes and root biology studies. As such it is an informative guide and experimental manual for researchers in diverse fields of plant biology.

About the authors

Dr. Vikas Srivastava is an Assistant Professor at the Department of Botany, Central University of Jammu, India. He completed his Ph.D. at Lucknow University, India, and his post-doc at the National Institute of Plant Genome Research (NIPGR), New Delhi, India. Specializing in various areas of biotechnology, he has training and experience in diverse fields of plant biology. He has published numerous articles in respected international journals/books of repute, and has been working as Principal Investigator for a major project sponsored by the University Grant Commission (UGC), New Delhi. He is a recipient of various awards and prestigious fellowships, and has delivered several invited lectures at leading national institutes.

Dr. Shakti Mehrotra is a consulting scientist at the Department of Biotechnology, Institute of Engineering and Technology, Dr. Abdul Kalam Technical University, Lucknow, India. She completed her Ph.D. at Lucknow University, India, and post-doc at the Institute of Engineering and Technology, Lucknow (DBT-PDF) and CSIR-CIMAP (DSTYoung Scientist). Specializing in various areas of biotechnology, she has training and experience in diverse fields of plant biotechnology. She has published numerous articles in respected national and international journals/books and worked as Principal Investigator for major projects sponsored by the Department of Science and Technology (DST) and Department of Biotechnology (DBT), Government of India. She is a recipient of various awards and prestigious fellowships. She has delivered several presentations at conferences and workshops.

Dr. Sonal Mishra is currently working as a Dr. D. S. Kothari-Post Doctoral Fellow (UGC-DSKPDF) at the School of Biotechnology, University of Jammu, India. She has recently completed a SERB-National Post Doc Fellowship (SERB-NPDF) as Principal Investigator at the School of Biotechnology, University of Jammu, India. Further, she completed a post-doc at Jawaharlal Nehru University and National Institute of Plant Genome Research (NIPGR), India. She pursued her Ph.D. jointly from the Central Institute of Medicinal and Aromatic Plants (CSIR-CIMAP, Lucknow) and Jawaharlal Nehru University, New Delhi. Specializing in various areas of biotechnology, she has training and experience in diverse fields of plant biotechnology and molecular biology. She has published numerous articles in respected international journals/books. She is a recipient of various awards and prestigious fellowships, and has presented her work in at seminars and conferences.



Table of contents (15 chapters)

Table of contents (15 chapters)
  • Hairy Roots Biotechnology Unzipped: A Journey of Reality and Promises

    Pages 1-10

    Mehrotra, Shakti (et al.)

  • Hairy Roots as a Source of Tropane Alkaloids

    Pages 11-42

    Skała, Ewa (et al.)

  • Induction, Metabolite Analysis, and Transgenesis of Hairy Roots from Coleus forskohlii

    Pages 43-54

    Jamwal, Vijay Lakshmi (et al.)

  • Establishment of Hairy Roots of Endangered Himalayan Plant Swertia chirata: A Sustainable Alternative to Extraction from Nature

    Pages 55-63

    Chouhan, Rekha (et al.)

  • A Protocol for the Selection of Spontaneous Variants from Established Catharanthus roseus Hairy Root Cultures

    Pages 65-75

    Vázquez-Flota, Felipe A. (et al.)

Buy this book

eBook $139.00
price for USA in USD
  • ISBN 978-981-15-4055-4
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $179.99
price for USA in USD
  • ISBN 978-981-15-4054-7
  • Free shipping for individuals worldwide
  • Immediate ebook access, if available*, with your print order
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Hairy Root Cultures Based Applications
Book Subtitle
Methods and Protocols
Editors
  • Vikas Srivastava
  • Shakti Mehrotra
  • Sonal Mishra
Series Title
Rhizosphere Biology
Copyright
2020
Publisher
Springer Singapore
Copyright Holder
Springer Nature Singapore Pte Ltd.
eBook ISBN
978-981-15-4055-4
DOI
10.1007/978-981-15-4055-4
Hardcover ISBN
978-981-15-4054-7
Series ISSN
2523-8442
Edition Number
1
Number of Pages
XIV, 237
Number of Illustrations
8 b/w illustrations, 39 illustrations in colour
Topics

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