Springer Theses

Synthesis and Biological Evaluation of Pyrrole–Imidazole Polyamide Probes for Visualization of Telomeres

Authors: Kawamoto, Yusuke

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  • Nominated by Kyoto University as an outstanding Ph.D. thesis
  • Includes a review chapter summarizing the history of and recent studies on Py–Im
  • Describes in detail the synthesis of Py–Im polyamides using an automatic solid-phase peptide
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eBook $84.99
price for USA in USD (gross)
  • ISBN 978-981-13-6912-4
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $149.99
price for USA in USD
  • ISBN 978-981-13-6911-7
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

This book presents a novel method of synthesizing sequence-specific DNA binding pyrrole–imidazole (Py–Im) polyamides and demonstrates polyamides targeting longer base pairs than those previously reported and their cellular application. It discusses the development of the facile synthetic method of tandem hairpin Py–Im polyamides and the method of visualizing telomeres under mild conditions without harsh denaturation, as well as research resulting in the synthesis not only of previously reported tandem dimer polyamides but also longer tandem trimer and tetramer polyamides. Using fluorescently labeled tandem hairpin Py–Im polyamides targeting a human telomere repetitive sequence, the author successfully stained telomeres in fixed cells without denaturation. These fluorescently labeled tandem tetramer polyamides are able to visualize human telomeres with lower background signals than previous polyamide probes, suggesting that they have higher specificity for telomeres. As such, this book provides insights into recent advance in Py–Im polyamides, particularly the extension of the target sites c

Table of contents (4 chapters)

Table of contents (4 chapters)
  • Introduction: Sequence-Specific DNA Binding Pyrrole–Imidazole Polyamides and Their Applications

    Kawamoto, Yusuke

    Pages 1-41

  • Development of a New Method for Synthesis of Tandem Hairpin Pyrrole–Imidazole Polyamide Probes Targeting Human Telomeres

    Kawamoto, Yusuke

    Pages 43-66

  • Tandem Trimer Pyrrole-Imidazole Polyamide Probes Targeting 18 Base Pairs in Human Telomere Sequences

    Kawamoto, Yusuke

    Pages 67-87

  • Targeting 24 bp Within Telomere Repeat Sequences with Tandem Tetramer Pyrrole–Imidazole Polyamide Probes

    Kawamoto, Yusuke

    Pages 89-119

Buy this book

eBook $84.99
price for USA in USD (gross)
  • ISBN 978-981-13-6912-4
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $149.99
price for USA in USD
  • ISBN 978-981-13-6911-7
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Synthesis and Biological Evaluation of Pyrrole–Imidazole Polyamide Probes for Visualization of Telomeres
Authors
Series Title
Springer Theses
Copyright
2019
Publisher
Springer Singapore
Copyright Holder
Springer Nature Singapore Pte Ltd.
eBook ISBN
978-981-13-6912-4
DOI
10.1007/978-981-13-6912-4
Hardcover ISBN
978-981-13-6911-7
Series ISSN
2190-5053
Edition Number
1
Number of Pages
XII, 122
Number of Illustrations
10 b/w illustrations, 52 illustrations in colour
Topics