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Multifaceted Protocol in Biotechnology

  • Gives many latest techniques in Biotechnology research with step by step procedure so that reader with directly use it as the laboratory manual

  • Table and color figures are from true experiments which clear reader to see what they should expect from each experiment

  • It touches many areas in biotechnology and bioprocess engineering therefore, the reader may virtualize the upstream and downstream experiments

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Table of contents (19 chapters)

  1. Front Matter

    Pages i-viii
  2. Site-Directed Mutagenesis on Plasmid Using Polymerase Chain Reaction

    • Abdul Aziz Ahmad, Hamzah Mohd Salleh, Ibrahim Ali Noorbatcha
    Pages 1-11
  3. Isolation of Halophilic Bacteria

    • Nadiah Syuhada Abd. Samad, Azura Amid
    Pages 13-22
  4. Construction of Metagenomic DNA Libraries and Enrichment Strategies

    • Farah Fadwa Benbelgacem, Hamzah Mohd. Salleh, Ibrahim Ali Noorbatcha
    Pages 23-42
  5. Thermotolerant Bacteria Producing Fibrinolytic Enzyme

    • Azura Amid, Nurul Aqilah Ab. Shukor
    Pages 43-51
  6. Chemical Mutation Method for High CO2-Requiring-Mutants of the Cyanobacterium Synechococcus sp. PCC 7002

    • Ummi Syuhada Halmi Shari, Azlin Suhaida Azmi, Azura Amid
    Pages 53-62
  7. Characterization of Electrochemical Transducers for Biosensor Applications

    • Farrah Aida Arris, Abdel Mohsen Benoudjit, Fahmi Sanober, Wan Wardatul Amani Wan Salim
    Pages 119-137
  8. Polymers in Biosensors

    • Jia Jia Long, Abdel Mohsen Benoudjit, Farrah Aida Arris, Fathilah Ali, Wan Wardatul Amani Wan Salim
    Pages 151-165
  9. Skim Latex Serum as an Alternative Nutrition for Microbial Growth

    • Vivi Mardina, Faridah Yusof
    Pages 179-196
  10. Method for Isolation of Bacterial Strain from Contaminated Soil for Biodegradation of Polycyclic Aromatic Hydrocarbons (PHAs)

    • Nooraidah Binti Mustaffa, Parveen Jamal, Kola Saheed Olorunnisola, Abdul Haseeb Ansari
    Pages 197-202
  11. Monitoring the Growth of Plant Cells in Suspension Culture

    • Noor Illi Mohamad Puad, Tajul Afif Abdullah
    Pages 203-214
  12. Culturing and Maintaining Mammalian Cell Culture

    • Raha Ahmad Raus, Ghassan Ali Salih, Mustaffa Nameer Shaban
    Pages 215-226
  13. Integrated Data Analysis Model for Screening Cell Line Producer

    • Nor Fadhillah Mohamed Azmin
    Pages 227-234

About this book

This edited work presents useful methods in experimenting in the area of Bioprocessing and Biotechnology. The four sections cover the area of Bioprocess, Whole Cells & Isolated Biocatalyst, Characterization of Biochemical Products and Cell Isolation & Culture. Its enable researchers to choose a suitable method and plan their experiments in details.

The main focus of this book is to provide step by step method to young researchers, especially in the new research areas. Among the latest areas are the isolation of novel strain or enzyme by metagenomic approaches and taming procedure in the laboratories, development of novel, the cheap and non-toxic catalyst for biodiesel production, and production of micro-fibrillated cellulose. An updated method for well-known areas such as immobilization technology, biosensor, and polymerization was also presented. The book also covers in-silico methods such as MATLAB platform to ease researchers. Not to forget, the method in animal and plant culture is also discussed in detail.

The book is written by chapter authors with much expertise in their fields. They have published multiple articles in the index listed journals.

The topic of this book is particularly relevant to young researchers who are struggling to fine-tune their research and do not want to waste their time in optimizing the experiment set up.


Editors and Affiliations

  • International Institute for Halal Research and Training (INHART), International Islamic University Malaysia, Kuala Lumpur, Malaysia

    Azura Amid

  • Department of Biotechnology Engineering, Kulliyyah of Engineering, International Islamic University Malaysia, Kuala Lumpur, Malaysia

    Sarina Sulaiman, Dzun Noraini Jimat, Nor Fadhillah Mohamed Azmin

About the editors

Dr. AzuraBinti Amid, a former graduate of the University of Malaya, Kuala Lumpur started her career as a Teacher at Segamat, Johor in 1996. She was appointed as an Assistant Lecturer at the International Islamic University Malaysia in 2001 and later as lecturer atKulliyyah of Engineering (IIUM) in 2011. She obtained the Bachelor of Science with Education (Biology),Master of Science (Molecular Biology in Plant) and Degree of Doctor of Philosophy in Molecular Biology from The University of London, United Kigdom. Currently she is attached as Assoc. Prof. to the Department of Biotechnology Engineering, International Islamic University Malaysia, Kuala Lumpur and Dean of Kulliyyah of Engineering, IIUM.

Bibliographic Information

  • Book Title: Multifaceted Protocol in Biotechnology

  • Editors: Azura Amid, Sarina Sulaiman, Dzun Noraini Jimat, Nor Fadhillah Mohamed Azmin

  • DOI: https://doi.org/10.1007/978-981-13-2257-0

  • Publisher: Springer Singapore

  • eBook Packages: Biomedical and Life Sciences, Biomedical and Life Sciences (R0)

  • Copyright Information: Springer Nature Singapore Pte Ltd. 2018

  • Hardcover ISBN: 978-981-13-2256-3Published: 04 February 2019

  • eBook ISBN: 978-981-13-2257-0Published: 23 January 2019

  • Edition Number: 1

  • Number of Pages: VIII, 244

  • Number of Illustrations: 65 b/w illustrations, 50 illustrations in colour

  • Topics: Protein Science

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access