Springer Theses

Study of Bacteriorhodopsin in a Controlled Lipid Environment

Authors: Yeh, Vivien

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  • Nominated by National Taiwan University as an outstanding Ph.D. thesis
  • Includes a detailed protocol for preparing nanodisc samples, as well as  for systematic study and careful sample optimization
  • Provides detailed insights into the effect of lipid composition on the function of an integral membrane protein by monitoring the photocycle kinetics initiated by pulsed illumination
  • Introduces an efficient method for direct assembly of native nanodiscs from biological membranes with production yield suitable for biophysical characterization, which can preserve both protein conformation and lipid composition
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eBook $109.00
price for USA in USD
  • ISBN 978-981-13-1238-0
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Hardcover $139.99
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Softcover $139.99
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About this book

This book focuses on the study of how the properties of nanodiscs, such as lipid composition and size, influence the function of the embedding integral membrane protein, bacteriorhodopsin. The author performed systematic studies to show that the lipid composition and the charge of the hydrophobic head and the structure of hydrophilic tails affect the photocycle pathway of bacteriorhodopsin, which is closely associated with its proton-pumping activity. Furthermore, the author demonstrated a highly efficient method for extracting membrane proteins directly from the biological membrane, preserving protein conformation, function and essential native lipids. This book demonstrates optimization and sample preparation, and presents practical methods of preparing membrane protein-embedded nanodisc samples for biophysical studies, which benefit structural and functional studies in the field of membrane protein characterization, both.

Table of contents (6 chapters)

Table of contents (6 chapters)

Buy this book

eBook $109.00
price for USA in USD
  • ISBN 978-981-13-1238-0
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • Immediate eBook download after purchase and usable on all devices
  • Bulk discounts available
Hardcover $139.99
price for USA in USD
Softcover $139.99
price for USA in USD
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Bibliographic Information

Bibliographic Information
Book Title
Study of Bacteriorhodopsin in a Controlled Lipid Environment
Authors
Series Title
Springer Theses
Copyright
2019
Publisher
Springer Singapore
Copyright Holder
Springer Nature Singapore Pte Ltd.
eBook ISBN
978-981-13-1238-0
DOI
10.1007/978-981-13-1238-0
Hardcover ISBN
978-981-13-1237-3
Softcover ISBN
978-981-13-4589-0
Series ISSN
2190-5053
Edition Number
1
Number of Pages
XVIII, 139
Number of Illustrations
18 b/w illustrations, 64 illustrations in colour
Topics