- Comprehensively summarizing common and advanced research protocols for Yersinia pestis research
- Including protocols for genomic and transcriptomic analysis, small RNA study, protein-protein interaction, immune response assays and animal models
- Providing online resources for Yersinia pestis research
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- About this book
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This protocol book includes common and advanced research protocols for Yersinia pestis research and for its identification, genotyping and source-tracing. This book includes protocols for genomic and transcriptomic analysis, small RNA study, protein-protein interaction, gene deletion. It will be helpful for graduate students, clinicians, and researchers in infectious diseases, systems biology, and evolution and numerous other relevant fields.
- About the authors
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Dr. Ruifu Yang is a professor at the Beijing Institute of Microbiology and Epidemiology and the Chief-Scientist for National Basic Research Program (973 Program). He serves as the vice president for the Chinese Society for Microbiology. His research focuses on bacterial pathogenesis using genomics- and proteomics-based techniques. He has published more than 100 articles on international well-known journals, such as Nature Genetics, PNAS, BMC Microbiology.
- Table of contents (33 chapters)
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Isolation and Identification of Yersinia pestis
Pages 1-14
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Isolation and Characterization of Yersinia pestis Bacteriophages
Pages 15-22
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Extraction of Chromosomal DNA and Plasmid from Yersinia pestis
Pages 23-27
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Extraction of Total RNA from Yersinia pestis
Pages 29-34
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Genotyping of Yersinia pestis
Pages 35-59
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Table of contents (33 chapters)
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Bibliographic Information
- Bibliographic Information
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- Book Title
- Yersinia Pestis Protocols
- Editors
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- Ruifu Yang
- Series Title
- Springer Protocols Handbooks
- Copyright
- 2018
- Publisher
- Springer Singapore
- Copyright Holder
- Springer Nature Singapore Pte Ltd.
- eBook ISBN
- 978-981-10-7947-4
- DOI
- 10.1007/978-981-10-7947-4
- Hardcover ISBN
- 978-981-10-7946-7
- Softcover ISBN
- 978-981-13-4024-6
- Series ISSN
- 1949-2448
- Edition Number
- 1
- Number of Pages
- VI, 290
- Number of Illustrations
- 26 b/w illustrations, 16 illustrations in colour
- Topics
