Overview
- Editors:
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Stanton B. Gelvin
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Purdue University, West Lafayette, USA
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Robbert A. Schilperoort
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Leiden University, Leiden, The Netherlands
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Table of contents (6 chapters)
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- Sangdun Choi, Rod A. Wing
Pages 1-28
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- Annemarie H. Meijer, Jan Schouten, Pieter B. F. Ouwerkerk, J. Harry C. Hoge
Pages 29-57
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- Henrik Albert, David W. Ow
Pages 59-73
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- L. Alexander Lyznik, David Peterson, Zuo-Yu Zhao, Xueni Guan, Ben Bowen, Bruce Drummond et al.
Pages 75-99
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- Christopher J. Staiger, Bryan C. Gibbon, Haiyun Ren
Pages 101-116
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- Chiyoko Machida, Hitoshi Onouchi, Endang Semiarti, Takaaki Ishikawa, Yasunori Machida
Pages 117-141
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Back Matter
Pages 143-167
About this book
Plant Molecular Biology Manual (Second Edition) is an entirely new manual containing both fundamental and recently described techniques in the area of plant molecular biology. Designed for use in the research laboratory, the Plant Molecular Biology Manual presents detailed techniques in the areas of plant transformation, recombinant DNA and other nucleic acid manipulations, nuclear run-on and in vitro transcription systems, in situ hybridization and immunodetection systems, protein-nucleic acid interaction analyses, subcellular targeting of proteins in the plant cell, and gene tagging using T-DNA and transposons. This second edition contains more than 40 newly written chapters, including descriptions of subjects such as virus-mediated gene transfer, specialized Agrobacterium strains and T-DNA vectors, nuclear run-on and in vitro transcription systems, non-radioactive detection systems, characterization of transcription factors, nuclear protein targeting, and T-DNA and transposon mutagenesis, not previously described in the first edition.
Reviews
About the first edition:
` Altogether, Plant Molecular Biology Manual is a very useful guide for the practical application of modern methods of molecular biology in plant sciences and is therefore highly recommended. '
Plant Breeding
` ...every researcher in plant molecular biology should take a look at this book '
New Phytologist
Editors and Affiliations
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Purdue University, West Lafayette, USA
Stanton B. Gelvin
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Leiden University, Leiden, The Netherlands
Robbert A. Schilperoort