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The Baculovirus Expression System

A laboratory guide

Authors: King, Linda

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  • ISBN 978-94-011-2374-7
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About this book

The decision to write a book about the practical aspects of the baculovirus expression system stems from the numerous phone calls for help we have had, and from the many visitors to our labora­ tories requiring assistance to find the elusive polyhedrin-negative virus containing their favourite gene. We have also organized two expression system workshops and from the manuals we wrote for these, it seemed a logical progression to extend them into book form. We appreciate that those who are 'old-hands' at the baculovirus expression system may have differing views on some of our procedures, but the methods in this book are presented in the light of our own experiences in the laboratory and from our practical workshops, and we hope that the book will be especially useful to those new to the system. The first three chapters give the background information to the baculovirus expression system, and includes advice on how to choose the right transfer vector and discusses the various methods that are available to select recombinant viruses. The practical chapters concentrate on those aspects which are novel to the baculovirus system (insect cell culture, virus amplification and titration, etc. ) and, in general, leave the standard molecular biological techniques to the other excellent laboratory manuals that are available. However, for completeness sake and to avoid constant reference to other manuals, we have included brief details of some standard techniques where they are integral to the success of the baculovirus protocols.

Table of contents (11 chapters)

Table of contents (11 chapters)

Buy this book

eBook $69.99
price for USA in USD (gross)
  • ISBN 978-94-011-2374-7
  • Digitally watermarked, DRM-free
  • Included format: PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Softcover $89.99
price for USA in USD
  • ISBN 978-94-010-5047-0
  • Free shipping for individuals worldwide
  • Immediate ebook access, if available*, with your print order
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
The Baculovirus Expression System
Book Subtitle
A laboratory guide
Authors
Copyright
1992
Publisher
Springer Netherlands
Copyright Holder
L. A. King and R. D. Possee
eBook ISBN
978-94-011-2374-7
DOI
10.1007/978-94-011-2374-7
Softcover ISBN
978-94-010-5047-0
Edition Number
1
Number of Pages
XIV, 229
Topics

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