Save 40% on books and eBooks in Engineering & Materials Science or in Social & Behavioral Sciences!

NATO Science for Peace and Security Series B: Physics and Biophysics

Biophysics and the Challenges of Emerging Threats

Editors: Puglisi, Joseph (Ed.)

Free Preview
  • Presents state of the art information on NMR spectroscopy, and its place in the broader field of biophysics
  • No other monograph presents such a wide range of topics, including NMR spectroscopy, protein folding, X-ray crystallography, spectroscopy and applications
see more benefits

Buy this book

eBook $84.99
price for USA in USD (gross)
  • ISBN 978-90-481-2368-1
  • Digitally watermarked, DRM-free
  • Included format: PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $249.99
price for USA in USD
  • ISBN 978-90-481-2366-7
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $109.00
price for USA in USD
  • ISBN 978-90-481-2367-4
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

Single-molecule techniques eliminate ensemble averaging, thus revealing transient or rare species in heterogeneous systems [1–3]. These approaches have been employed to probe myriad biological phenomena, including protein and RNA folding [4–6], enzyme kinetics [7, 8], and even protein biosynthesis [1, 9, 10]. In particular, immobilization-based fluorescence te- niques such as total internal reflection fluorescence microscopy (TIRF-M) have recently allowed for the observation of multiple events on the millis- onds to seconds timescale [11–13]. Single-molecule fluorescence methods are challenged by the instability of single fluorophores. The organic fluorophores commonly employed in single-molecule studies of biological systems display fast photobleaching, intensity fluctuations on the millisecond timescale (blinking), or both. These phenomena limit observation time and complicate the interpretation of fl- rescence fluctuations [14, 15]. Molecular oxygen (O) modulates dye stability. Triplet O efficiently 2 2 quenches dye triplet states responsible for blinking. This results in the for- tion of singlet oxygen [16–18]. Singlet O reacts efficiently with organic dyes, 2 amino acids, and nucleobases [19, 20]. Oxidized dyes are no longer fluor- cent; oxidative damage impairs the folding and function of biomolecules. In the presence of saturating dissolved O , blinking of fluorescent dyes is sup- 2 pressed, but oxidative damage to dyes and biomolecules is rapid. Enzymatic O -scavenging systems are commonly employed to ameliorate dye instability. 2 Small molecules are often employed to suppress blinking at low O levels.

Table of contents (8 chapters)

Table of contents (8 chapters)

Buy this book

eBook $84.99
price for USA in USD (gross)
  • ISBN 978-90-481-2368-1
  • Digitally watermarked, DRM-free
  • Included format: PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $249.99
price for USA in USD
  • ISBN 978-90-481-2366-7
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $109.00
price for USA in USD
  • ISBN 978-90-481-2367-4
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Loading...

Recommended for you

Loading...

Bibliographic Information

Bibliographic Information
Book Title
Biophysics and the Challenges of Emerging Threats
Editors
  • Joseph Puglisi
Series Title
NATO Science for Peace and Security Series B: Physics and Biophysics
Copyright
2009
Publisher
Springer Netherlands
Copyright Holder
Springer Science+Business Media B.V.
eBook ISBN
978-90-481-2368-1
DOI
10.1007/978-90-481-2368-1
Hardcover ISBN
978-90-481-2366-7
Softcover ISBN
978-90-481-2367-4
Series ISSN
1874-6500
Edition Number
1
Number of Pages
VII, 179
Topics