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Springer Theses

Applications of Aminoacylation Ribozymes That Recognize the 3′-end of tRNA

Authors: Terasaka, Naohiro

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  • Nominated by The University of Tokyo as an outstanding Ph.D. thesis
  • Provides a detailed description of the history and applications of aminoacylation ribozymes
  • Describes potentials of aminoacylation ribozymes to be applied for further study
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  • ISBN 978-4-431-56517-8
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About this book

In this thesis, applications of aminoacylation ribozymes named flexizymes are described. Flexizymes have the following unique characteristics: (i) substrate RNA is recognized by two consecutive base pairs between the 3'-end of substrate RNA and the 3'-end of the flexizyme; (ii) these base pairs can be substituted with other base pairs; and (iii) various activated amino acids can be used as substrates including both canonical and noncanonical amino acids. This flexible aminoacylation of RNAs by flexizymes was used to label endogenous tRNAs to be removed, and in vitro selection using the tRNA-depleted library enabled the discovery of the novel interaction between the microRNA precursor and metabolites. Flexizymes are also used to prepare various aminoacyl-tRNAs bearing mutations at the 3'-end to engineer the translation machinery and to develop the orthogonal translation machinery.
The first part of the research demonstrated that SELEX is appropriate for discovering the interaction between small RNA and ligands, and suggested that more RNA motif binding to small molecules exists in small RNAs. The second part opened a door to new opportunities for in vitro synthetic biology involving the engineering of the genetic codes and translation machineries. This research also indicated the great potential of aminoacylation by flexizymes to be applied in various fields of RNA research, which is beneficial for RNA researchers.

Table of contents (4 chapters)

Table of contents (4 chapters)

Buy this book

eBook $109.00
price for USA in USD
  • ISBN 978-4-431-56517-8
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $139.99
price for USA in USD
Softcover $139.99
price for USA in USD
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Bibliographic Information

Bibliographic Information
Book Title
Applications of Aminoacylation Ribozymes That Recognize the 3′-end of tRNA
Authors
Series Title
Springer Theses
Copyright
2017
Publisher
Springer Japan
Copyright Holder
Springer Japan KK, part of Springer Nature
eBook ISBN
978-4-431-56517-8
DOI
10.1007/978-4-431-56517-8
Hardcover ISBN
978-4-431-56515-4
Softcover ISBN
978-4-431-56809-4
Series ISSN
2190-5053
Edition Number
1
Number of Pages
XII, 89
Number of Illustrations
10 b/w illustrations, 28 illustrations in colour
Topics