Analytical Ultracentrifugation
Instrumentation, Software, and Applications
Editors: Uchiyama, S., Arisaka, F., Stafford, W.F., Laue, T. (Eds.)
Free Preview- Covers recent advances in AUC studies comprehensively
- Includes knowledge on hardware, software, and applications
- Provides essential knowledge for readers who are engaged in the practical use of AUC in industries
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- About this book
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This book introduces analytical ultracentrifugation (AUC) as a whole, covering essential theoretical and practical aspects as well as its applications in both biological and non-biological systems. Comprehensive characterizations of macromolecules in a solution are now routinely required not only for understanding the solution system but also for producing a solution with better properties. Analytical ultracentrifugation is one of most powerful and reliable techniques for studying the biophysical behavior of solutes in solution. In the last few years, there have been steady advances made in hardware, software, and applications for AUC.
This book provides chapters that cover everything essential for beginners to the most advanced users and also offer updated knowledge of the field on advances in hardware, software, and applications. Recent development of hardware described in this book covers new detection systems that give added dimensions to AUC. Examples of data analysis with essential theoretical explanations for advanced and recently updated software are also introduced. Besides AUC of biological systems including membrane proteins and biopharmaceuticals, AUC applications for non-biological questions are included. AUC studies under non-ideal conditions such as highly concentrated solutions and solutions with high salt concentration are also included.
The contributors to this book are leading researchers in the fields of solution biophysics and physical chemistry who extensively employ AUC analysis for their research. From this published work, one can gain new and comprehensive knowledge of recent AUC analysis. - About the authors
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Susumu Uchiyama, Ph. D., Associate Professor, Department of Biotechnology, Graduate School of Engineering, Osaka University, 2‐1 Yamadaoka, Suita, Osaka 565‐0871, Japan
Fumio Arisaka, Ph. D., Professor, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta, Midori‐ku, Yokohama 226‐8501, Japan
Walter F. Stafford, III, Ph.D., Department of Neurology, Harvard Medical School, Boston, Massachusetts 02115, USA
Tom Laue, Ph.D., Professor, Biomolecular Interaction Technologies Center (BITC), Center to Advance Molecular Interaction Science (CAMIS), University of New Hampshire, Rudman Hall, Room 379, 46 CollegeRoad, Durham, NH 03824, USA
- Table of contents (26 chapters)
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Important and Essential Theoretical Aspects of AUC
Pages 3-14
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Experimental Design and Practical Aspects
Pages 15-21
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The CFA Analytical Ultracentrifuge Architecture
Pages 25-37
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Fluorescence Detection System
Pages 39-61
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The Multiwavelength UV/Vis Detector: New Possibilities with an Added Spectral Dimension
Pages 63-80
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Table of contents (26 chapters)
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Bibliographic Information
- Bibliographic Information
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- Book Title
- Analytical Ultracentrifugation
- Book Subtitle
- Instrumentation, Software, and Applications
- Editors
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- Susumu Uchiyama
- Fumio Arisaka
- Walter F. Stafford
- Tom Laue
- Copyright
- 2016
- Publisher
- Springer Japan
- Copyright Holder
- Springer Japan
- eBook ISBN
- 978-4-431-55985-6
- DOI
- 10.1007/978-4-431-55985-6
- Hardcover ISBN
- 978-4-431-55983-2
- Softcover ISBN
- 978-4-431-56745-5
- Edition Number
- 1
- Number of Pages
- IX, 532
- Number of Illustrations
- 61 b/w illustrations, 117 illustrations in colour
- Topics
