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Electroporation and Sonoporation in Developmental Biology

Editors: Nakamura, Harukazu (Ed.)

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  • ISBN 978-4-431-09427-2
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About this book

In ovo electroporation is an epoch-making achievement in the study of developmental biology. With this method, experiments can be carried out in gain and loss of function in desired tissue at any desired stage in chick embryos. Introduction of a tetracycline-regulated gene expression system and a transposon system has further extended the potential of the method, making it possible to obtain long-term expression and to turn on and off a gene of interest. It is now applied to mice, aquatic animals, and even to plants for the study of developmental biology and for other purposes. In this book, the application of electroporation in many embryonic tissues and organs is introduced, with some chapters that deal with gene transfer in adults. Sonoporation, another useful tool, using ultrasonic waves instead of electric currents, for gene transfer to mesenchymal tissues is also introduced.

Table of contents (27 chapters)

Table of contents (27 chapters)
  • Short History of Electroporation for the Study of Developmental Biology

    Pages 3-7

    Nakamura, Harukazu, Phd

  • In Ovo Electroporation as a Useful Tool to Pursue Molecular Mechanisms of Neural Development in Chick Embryos

    Pages 9-16

    Odani, Noritaka (et al.)

  • Applications of Tol2 Transposon-Mediated Gene Transfer for Stable Integration and Conditional Expression of Electroporated Genes in Chicken Embryos

    Pages 17-24

    Sato, Yuki (et al.)

  • Temporal Control of Gene Expression by Combining Electroporation and the Tetracycline Inducible Systems in Vertebrate Embryos

    Pages 25-36

    Dubrulle, Julien (et al.)

  • In Ovo Electroporation for Targeting the Somitic Mesoderm

    Pages 37-42

    Ohata, Emi (et al.)

Buy this book

eBook $129.00
price for USA in USD (gross)
  • ISBN 978-4-431-09427-2
  • Digitally watermarked, DRM-free
  • Included format: PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Softcover $169.99
price for USA in USD
  • ISBN 978-4-431-09426-5
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Electroporation and Sonoporation in Developmental Biology
Editors
  • Harukazu Nakamura
Copyright
2009
Publisher
Springer Japan
Copyright Holder
Springer-Verlag Tokyo
eBook ISBN
978-4-431-09427-2
DOI
10.1007/978-4-431-09427-2
Softcover ISBN
978-4-431-09426-5
Edition Number
1
Number of Pages
XXIX, 331
Topics