Springer Protocols Handbooks Hydrocarbon and Lipid Microbiology Protocols

Hydrocarbon and Lipid Microbiology Protocols

Meso- and Microcosms

Editors: McGenity, Terry J., Timmis, Kenneth N., Nogales Fernández, Balbina (Eds.)

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  • Offers readily reproducible, step-by-step laboratory methods
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About this book

This Volume describes methods for simulating natural environments by using reproducible and controllable meso- and microcosm experiments to analyse hydrocarbon-degrading microorganisms and to test hypotheses. It presents important aspects of the preparation of experimental set-ups, hydrocarbon application and sampling, and features protocols for experiments with different types of samples, such as biofilms, aquatic systems (e.g. groundwater, streams), and sediments, including oscillating oxic-anoxic mesocosms, as well as for in-situ experimentation in subtidal and deep sediments. Two chapters are dedicated to cultivation under high-pressure conditions, and several chapters include protocols for processing samples for downstream chemical, microbial or activity analyses. Several of the approaches presented are generic and will benefit anyone embarking on designing meso- and microcosm experiments. <

Hydrocarbon and Lipid Microbiology Protocols

There are tens of thousands of structurally different hydrocarbons, hydrocarbon derivatives and lipids, and a wide array of these molecules are required for cells to function. The global hydrocarbon cycle, which is largely driven by microorganisms, has a major impact on our environment and climate. Microbes are responsible for cleaning up the environmental pollution caused by the exploitation of hydrocarbon reservoirs and will also be pivotal in reducing our reliance on fossil fuels by providing biofuels, plastics and industrial chemicals. Gaining an understanding of the relevant functions of the wide range of microbes that produce, consume and modify hydrocarbons and related compounds will be key to responding to these challenges. This comprehensive collection of current and emerging protocols will facilitate acquisition of this understanding and exploitation of useful activities of such microbes.





Table of contents (12 chapters)

Table of contents (12 chapters)
  • Introduction: Mesocosms and Microcosms

    Pages 1-13

    Prince, Roger C.

  • Preparing the Hydrocarbon/Crude Oil

    Pages 15-32

    Prince, Roger C. (et al.)

  • High Pressure Cultivation of Hydrocarbonoclastic Aerobic Bacteria

    Pages 33-49

    Smedile, Francesco (et al.)

  • In Vitro High-Pressure Incubation and Activity Measurement of Deep-Sea Methanogenic Archaea

    Pages 51-64

    Tasumi, Eiji (et al.)

  • Bioreactor Design to Emulate Deep-Sea Hydrocarbon Releases Including Formation of Gas Hydrates

    Pages 65-78

    Nikolopoulou, Maria (et al.)

Buy this book

eBook $119.00
price for USA in USD
  • ISBN 978-3-662-53108-2
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $159.99
price for USA in USD
  • ISBN 978-3-662-53107-5
  • Free shipping for individuals worldwide
  • Institutional customers should get in touch with their account manager
  • Covid-19 shipping restrictions
  • Usually ready to be dispatched within 3 to 5 business days, if in stock
Softcover $159.99
price for USA in USD
  • ISBN 978-3-662-57112-5
  • Free shipping for individuals worldwide
  • Institutional customers should get in touch with their account manager
  • Covid-19 shipping restrictions
  • Usually ready to be dispatched within 3 to 5 business days, if in stock
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Bibliographic Information

Bibliographic Information
Book Title
Hydrocarbon and Lipid Microbiology Protocols
Book Subtitle
Meso- and Microcosms
Editors
  • Terry J. McGenity
  • Kenneth N. Timmis
  • Balbina Nogales Fernández
Series Title
Springer Protocols Handbooks
Copyright
2017
Publisher
Springer-Verlag Berlin Heidelberg
Copyright Holder
Springer-Verlag GmbH Germany, part of Springer Nature
eBook ISBN
978-3-662-53108-2
DOI
10.1007/978-3-662-53108-2
Hardcover ISBN
978-3-662-53107-5
Softcover ISBN
978-3-662-57112-5
Series ISSN
1949-2448
Edition Number
1
Number of Pages
X, 173
Number of Illustrations
13 b/w illustrations, 27 illustrations in colour
Topics