Editors:
- Offers readily reproducible, step-by-step laboratory methods
- Provides helpful tips and tricks to complement the protocols
- Includes troubleshooting advice
- Features practical laboratory guidelines to promote successful results
Part of the book series: Springer Protocols Handbooks (SPH)
Buy it now
Buying options
Tax calculation will be finalised at checkout
Other ways to access
This is a preview of subscription content, log in via an institution to check for access.
Table of contents (12 protocols)
-
Front Matter
About this book
This Volume describes methods for simulating natural environments by using reproducible and controllable meso- and microcosm experiments to analyse hydrocarbon-degrading microorganisms and to test hypotheses. It presents important aspects of the preparation of experimental set-ups, hydrocarbon application and sampling, and features protocols for experiments with different types of samples, such as biofilms, aquatic systems (e.g. groundwater, streams), and sediments, including oscillating oxic-anoxic mesocosms, as well as for in-situ experimentation in subtidal and deep sediments. Two chapters are dedicated to cultivation under high-pressure conditions, and several chapters include protocols for processing samples for downstream chemical, microbial or activity analyses. Several of the approaches presented are generic and will benefit anyone embarking on designing meso- and microcosm experiments. <
Hydrocarbon and Lipid Microbiology Protocols
There are tens of thousands of structurally different hydrocarbons, hydrocarbon derivatives and lipids, and a wide array of these molecules are required for cells to function. The global hydrocarbon cycle, which is largely driven by microorganisms, has a major impact on our environment and climate. Microbes are responsible for cleaning up the environmental pollution caused by the exploitation of hydrocarbon reservoirs and will also be pivotal in reducing our reliance on fossil fuels by providing biofuels, plastics and industrial chemicals. Gaining an understanding of the relevant functions of the wide range of microbes that produce, consume and modify hydrocarbons and related compounds will be key to responding to these challenges. This comprehensive collection of current and emerging protocols will facilitate acquisition of this understanding and exploitation of useful activities of such microbes.
Editors and Affiliations
-
School of Biological Sciences, University of Essex, Colchester, United Kingdom
Terry J. McGenity
-
Braunschweig, Germany
Kenneth N. Timmis
-
Dept. Biologia, University of the Balearic Islands and Mediterranean Institute for Advanced Studies (IMEDEA, UIB-CSIC), Palma de Mallorca, Spain
Balbina Nogales
Bibliographic Information
Book Title: Hydrocarbon and Lipid Microbiology Protocols
Book Subtitle: Meso- and Microcosms
Editors: Terry J. McGenity, Kenneth N. Timmis, Balbina Nogales
Series Title: Springer Protocols Handbooks
DOI: https://doi.org/10.1007/978-3-662-53108-2
Publisher: Springer Berlin, Heidelberg
eBook Packages: Springer Protocols
Copyright Information: Springer-Verlag GmbH Germany, part of Springer Nature 2017
Hardcover ISBN: 978-3-662-53107-5Published: 26 June 2017
Softcover ISBN: 978-3-662-57112-5Published: 15 August 2018
eBook ISBN: 978-3-662-53108-2Published: 08 June 2017
Series ISSN: 1949-2448
Series E-ISSN: 1949-2456
Edition Number: 1
Number of Pages: X, 173
Number of Illustrations: 13 b/w illustrations, 27 illustrations in colour
Topics: Microbiology, Biochemical Engineering