Springer Protocols Handbooks Hydrocarbon and Lipid Microbiology Protocols

Hydrocarbon and Lipid Microbiology Protocols

Ultrastructure and Imaging

Editors: McGenity, Terry J., Timmis, Kenneth N., Fernandez, Balbina Nogales (Eds.)

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  • ISBN 978-3-662-49134-8
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About this book

This Volume presents key microscopy and imaging methods for revealing the structure and ultrastructure of environmental and experimental samples, of microbial communities and cultures, and of individual cells. Method adaptations that specifically address problems concerning the hydrophobic components of samples are highlighted and discussed. The methods described range from electron microscopy and light and fluorescence microscopy, to confocal laser-scanning microscopy, and include experimental set-ups for the analysis of interfacial processes like microbial growth and activities at hydrocarbon:water interfaces, biofilms and microbe:mineral interfaces. Three forms of fluorescence in situ hybridization - CARD-FISH, MAR-FISH and Two-pass TSA-FISH - are described for the ecophysiological analysis of functionally active microbes in samples. The methods presented will enable readers to obtain an ultrastructural picture of, and identify the key functional microbes in, samples under investigation. This in turn will constitute a key framework for the interpretation of information from other experimental approaches, such as physicochemical analyses and genomic investigations.
Hydrocarbon and Lipid Microbiology ProtocolsThere are tens of thousands of structurally different hydrocarbons, hydrocarbon derivatives and lipids, and a wide array of these molecules are required for cells to function. The global hydrocarbon cycle, which is largely driven by microorganisms, has a major impact on our environment and climate. Microbes are responsible for cleaning up the environmental pollution caused by the exploitation of hydrocarbon reservoirs and will also be pivotal in reducing our reliance on fossil fuels by providing biofuels, plastics and industrial chemicals. Gaining an understanding of the relevant functions of the wide range of microbes that produce, consume and modify hydrocarbons and related compounds will be key to responding to these challenges. This comprehensive collection of current and emerging protocols will facilitate acquisition of this understanding and exploitation of useful activities of such microbes.

Table of contents (11 chapters)

Table of contents (11 chapters)
  • Ultrastructure and Imaging

    Pages 1-3

    Auer, Manfred

  • Electron Microscopy Protocols for the Study of Hydrocarbon-Producing and Hydrocarbon-Decomposing Microbes: Classical and Advanced Methods

    Pages 5-28

    Jhamb, Kamna (et al.)

  • Protocol for Laser Scanning Microscopy of Microorganisms on Hydrocarbons

    Pages 29-47

    Neu, Thomas R. (et al.)

  • Fluorescence Microscopy for Microbiology

    Pages 49-69

    Molinari, Gabriella

  • Imaging Bacterial Cells and Biofilms Adhering to Hydrophobic Organic Compound–Water Interfaces

    Pages 71-83

    Canette, Alexis (et al.)

Buy this book

eBook $89.00
price for USA in USD
  • ISBN 978-3-662-49134-8
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $149.99
price for USA in USD
Softcover $119.00
price for USA in USD
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Bibliographic Information

Bibliographic Information
Book Title
Hydrocarbon and Lipid Microbiology Protocols
Book Subtitle
Ultrastructure and Imaging
Editors
  • Terry J. McGenity
  • Kenneth N. Timmis
  • Balbina Nogales Fernandez
Series Title
Springer Protocols Handbooks
Copyright
2016
Publisher
Springer-Verlag Berlin Heidelberg
Copyright Holder
Springer-Verlag Berlin Heidelberg
eBook ISBN
978-3-662-49134-8
DOI
10.1007/978-3-662-49134-8
Hardcover ISBN
978-3-662-49132-4
Softcover ISBN
978-3-662-56983-2
Series ISSN
1949-2448
Edition Number
1
Number of Pages
X, 174
Topics