Modern Methods in Forest Genetics

1. Front Matter

   Pages I-XIV

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2. Optical Techniques for Measuring DNA Quantity

   • G. P. Berlyn, R. A. Cecich

   Pages 1-18

3. Nucleic Acid Extraction, Purification, Reannealing, and Hybridization Methods

   • R. B. Hall, J. P. Miksche, K. M. Hansen

   Pages 19-48

4. Gel Electrophoresis of Proteins and Enzymes

   • P. P. Feret, F. Bergmann

   Pages 49-77

5. Extraction and Analysis of Free and Protein-Bound Amino Acids from Norway Spruce Foliage

   • J. Lunderstädt

   Pages 78-88

6. Photosynthesis, Respiration, and Dry Matter Production

   • W. Żelawski, R. B. Walker

   Pages 89-119

7. Analyses of Monoterpenes of Conifers by Gas-Liquid Chromatography

   • A. E. Squillace

   Pages 120-157

8. Isolation and Analysis of Plant Phenolics from Foliage in Relation to Species Characterization and to Resistance Against Insects and Pathogens

   • J. Lunderstädt

   Pages 158-164

9. Mineral Analyses

   • F. H. Evers, W. Bücking

   Pages 165-188

10. Pollution Responses

    • K. F. Jensen, L. S. Dochinger, B. R. Roberts, A. M. Townsend

    Pages 189-216

11. Indirect Selection for Improvement of Desired Traits

    • K. von Weissenberg

    Pages 217-228

12. Pollen Handling Techniques in Forest Genetics, with Special Reference to Incompatibility

    • E. G. Kirby, R. G. Stanley

    Pages 229-242

13. Tissue Culture of Trees

    • L. Winton, O. Huhtinen

    Pages 243-264

14. Manipulation of Flowering in Conifers Through the Use of Plant Hormones

    • R. P. Pharis

    Pages 265-282

15. Back Matter

    Pages 283-290

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The present volume contains papers developed from courses given at the International Union of Forest Research Organizations (IUFRO) Bio­ chemical Genetics Workshop (Working Party S.04-5) held at the Univer­ sity of Gottingen, Germany on July 5 through 28, 1973. The workshop was organized by Professor Robert G. Stanley and was held in memory of Professor Klaus Stern. Unfortunately, both met with untimely deaths. Professor Stanley was also instrumental in initiating the process of having the workshop proceedings published. I was asked by the workshop participants to complete this task, and I wish to acknowledge their cooperation, advice and encouragement. In addition to the courses and subsequent papers resulting from the above workshop, we have included some papers by colleagues who were unable to attend the meeting. The contents of this text may, there­ fore, be considered a working-manual of generally "modern" techniques that are applicable to forest genetics and breeding programs. The chapters are placed in five major categories. The first three categories follow according to classes of chemical constituents in­ herent to plants which are nucleic acids (DNA, RNA) , primary gene products (amino acids, proteins and enzymes) and primary and secon­ dary metabolites (carbohydrate polymers, resins, phenolics, pigments, etc.). The fourth category is concerned with the interaction of en­ vironment and gene systems. Indirect selection, crossing and proto­ plasmic and flowering manipulation are factors covered in the fifth category.

• forest
• genes
• genetics
• plants
• protein

• U.S.Department of Agriculture, Forest Service Institute of Forest Genetics, North Central Forest Experiment Station, Rhinelander, USA

  Jerome P. Miksche

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